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Differential loading methods for BMP-2 within injectable calcium phosphate cement.

Floor C J van de Watering1, Janneke D M Molkenboer-Kuenen, Otto C Boerman

  • 1Department of Biomaterials, Radboud University Nijmegen Medical Center, PO Box 9101, 6500 HB Nijmegen, The Netherlands.

Journal of Controlled Release : Official Journal of the Controlled Release Society
|July 18, 2012
PubMed
Summary
This summary is machine-generated.

Loading bone morphogenetic protein-2 (BMP-2) into calcium phosphate cement (CPC) via PLGA microparticles or liquid phase shows similar release profiles. Osteoinductivity may depend on initial burst release, impacting injectable CPC applications.

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Drug Delivery Systems

Background:

  • Injectable calcium phosphate cement (CPC) offers potential for bone regeneration but faces limitations in growth factor delivery methods.
  • Effective loading of bone morphogenetic protein-2 (BMP-2) is crucial for enhancing CPC's osteoinductive capabilities.

Purpose of the Study:

  • To evaluate in vitro and in vivo release kinetics of BMP-2 using different loading strategies in CPC.
  • To assess the osteoinductive capacity of CPC scaffolds with varied BMP-2 incorporation methods.
  • To compare BMP-2 release and biological response between PLGA microparticle loading, liquid phase incorporation, and surface adsorption.

Main Methods:

  • In vitro release experiments and in vivo microSPECT imaging with (125)I-labeled BMP-2 were employed.
  • BMP-2 was loaded onto poly(d,l-lactic-co-glycolic acid) (PLGA) microparticles, into the CPC liquid phase, or adsorbed to the surface of porous CPC.
  • Osteoinductive capacity was assessed in a subcutaneous rat model using generated scaffolds.

Main Results:

  • Loading BMP-2 via PLGA microparticles or the CPC liquid phase yielded similar 28-day release profiles, differing from surface-loaded controls.
  • A significantly decreased burst release was observed for PLGA microparticle and liquid phase loading compared to surface adsorption.
  • Only CPC scaffolds with surface-loaded BMP-2 demonstrated osteoinductive capacity, potentially linked to burst release differences.

Conclusions:

  • Loading BMP-2 into injectable CPC via PLGA microparticles or the liquid phase is feasible, offering controlled release profiles.
  • The initial burst release of BMP-2 significantly influences the osteoinductive potential of CPC scaffolds.
  • Further investigation is needed to understand how differential BMP-2 loading methods affect biological responses in bone regeneration settings.