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Published on: December 8, 2016
Development of bone marrow mesenchymal stem cell culture in vitro
Li Zhang1, Li-Pan Peng, Nan Wu
1Department of Gastrointestinal Surgery, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, China. pzzl@sina.com
Objective:
To review the in vitro development of bone marrow mesenchymal stem cells culture (BM-MSC).
Data Sources:
The data cited in this review were mainly obtained from articles listed in Medline and PubMed. The search terms were "bone marrow mesenchymal stem cell" and "cell culture".
Study Selection:
Articles regarding the in vitro development of BM-MSCs culture, as well as the challenge of optimizing cell culture environment in two-dimensional (2D) vs. 3D.
Results:
Improving the culture conditions increases the proliferation and reduces the differentiation. Optimal values for many culture parameters remain to be identified. Expansion of BM-MSCs under defined conditions remains challenging, including the development of optimal culture conditions for BMSC and large-volume production systems.
Conclusions:
Expansion of BM-MSCs under defined conditions remains challenges, including the development of optimal culture conditions for BMSC and scale-up to large-volume production systems. Optimal values for many culture parameters remain to be identified.
