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Related Concept Videos

Introduction to Enzymes01:22

Introduction to Enzymes

The use of enzymes by humans dates to 7000 BCE. Humans first used enzymes to ferment sugars and produce alcohol without knowing that this was an enzyme-catalyzed reaction. Wilhelm Kuhne coined the term 'enzyme' in 1877 from the Greek words ‘en’ meaning ‘in’ or ‘within’ and ‘zyme’ meaning ‘yeast.’
Most enzymes are proteins that speed up biochemical reactions without being consumed. Enzymes contain one or more active sites that bind the substrates and convert them into products. Many enzymes also...
Introduction To Enzymes01:22

Introduction To Enzymes

The use of enzymes by humans dates to 7000 BCE. Humans first used enzymes to ferment sugars and produce alcohol without knowing that this was an enzyme-catalyzed reaction. Wilhelm Kuhne coined the term 'enzyme' in 1877 from the Greek words ‘en’ meaning ‘in’ or ‘within’ and ‘zyme’ meaning ‘yeast.’
Most enzymes are proteins that speed up biochemical reactions without being consumed. Enzymes contain one or more active sites that bind the substrates and convert them into products. Many enzymes also...
Catalytically Perfect Enzymes01:07

Catalytically Perfect Enzymes

The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.
Introduction to Enzyme Kinetics01:19

Introduction to Enzyme Kinetics

Enzyme kinetics studies the rates of biochemical reactions. Scientists monitor the reaction rates for a particular enzymatic reaction at various substrate concentrations. Additional trials with inhibitors or other molecules that affect the reaction rate may also be performed.
The experimenter can then plot the initial reaction rate or velocity (Vo) of a given trial against the substrate concentration ([S]) to obtain a graph of the reaction properties. For many enzymatic reactions involving a...
Enzyme Kinetics01:19

Enzyme Kinetics

Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
Enzyme Inhibition01:30

Enzyme Inhibition

Inhibitors are molecules that reduce enzyme activity by binding to the enzyme. In a normally functioning cell, enzymes are regulated by a variety of inhibitors. Drugs and other toxins can also inhibit enzymes. Some inhibitors bind to the enzyme’s active site, while others inhibit enzymatic activity by binding to other sites on the protein structure.

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Related Experiment Video

Updated: May 20, 2026

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
08:10

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

[Preface for special issue on enzyme engineering (2012)].

Cheng Jin1

  • 1Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. jinc@im.ac.cn

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|July 19, 2012
PubMed
Summary
This summary is machine-generated.

Enzyme engineering, a cornerstone of biotechnology, is advancing artificial biosystems for biofuels. This special issue highlights new enzyme discovery, mechanisms, and applications to boost research in China.

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Protein Engineering by Yeast Surface Display
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Last Updated: May 20, 2026

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
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Published on: August 8, 2016

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Protein Engineering by Yeast Surface Display
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Protein Engineering by Yeast Surface Display

Published on: November 29, 2024

Area of Science:

  • Enzyme engineering as a multidisciplinary field integrating enzymology and engineering principles.
  • Its growing importance as a foundational technology for modern biotechnology and the bio-economy.

Background:

  • Recent advancements in synthetic biology enabling the development of artificial biosystems.
  • The pivotal role of enzyme engineering within synthetic biology research.
  • Increasing global attention and research focus on enzyme engineering.

Discussion:

  • This special issue of "Enzyme Engineering" aims to promote enzyme engineering research within China.
  • It features invited reviews and selected research articles.
  • The content focuses on key areas within the field.

Key Insights:

  • Exploration of novel enzyme discovery and characterization.
  • Understanding enzyme mechanisms of action.
  • Advancements in enzyme production and biotechnological applications.

Outlook:

  • Potential for enzyme engineering to drive innovation in biofuels and biorefineries.
  • Continued growth and impact of enzyme engineering in synthetic biology.
  • Fostering collaborative research and development in enzyme engineering globally.