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Related Concept Videos

Nuclear Fusion02:45

Nuclear Fusion

The process of converting very light nuclei into heavier nuclei is also accompanied by the conversion of mass into large amounts of energy, a process called fusion. The principal source of energy in the sun is a net fusion reaction in which four hydrogen nuclei fuse and ultimately produce one helium nucleus and two positrons.
A helium nucleus has a mass that is 0.7% less than that of four hydrogen nuclei; this lost mass is converted into energy during the fusion. This reaction produces about...
Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
Catalytically Perfect Enzymes01:07

Catalytically Perfect Enzymes

The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.
Introduction to Enzymes01:22

Introduction to Enzymes

The use of enzymes by humans dates to 7000 BCE. Humans first used enzymes to ferment sugars and produce alcohol without knowing that this was an enzyme-catalyzed reaction. Wilhelm Kuhne coined the term 'enzyme' in 1877 from the Greek words ‘en’ meaning ‘in’ or ‘within’ and ‘zyme’ meaning ‘yeast.’
Most enzymes are proteins that speed up biochemical reactions without being consumed. Enzymes contain one or more active sites that bind the substrates and convert them into products. Many enzymes also...

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Related Experiment Video

Updated: May 20, 2026

Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation
19:23

Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation

Published on: January 16, 2019

[Recent progress in fusion enzyme design and applications].

Ziliang Huang1, Chong Zhang, Xi Wu

  • 1Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|July 19, 2012
PubMed
Summary

Fusion enzymes enhance industrial biocatalysis through molecular engineering. This review details design strategies, linker effects, and applications, highlighting challenges and future directions for enzyme technology.

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Area of Science:

  • Biotechnology
  • Enzyme Engineering
  • Industrial Biocatalysis

Background:

  • Enzyme engineering is crucial for industrial biocatalysis.
  • Fusion enzyme technology utilizes fusion protein design for enzyme engineering.
  • This approach enables multifunctional enzyme construction and precise enzyme proximity control.

Purpose of the Study:

  • To review recent advancements in molecular design strategies for fusion enzymes.
  • To systematically summarize the design principles and applications of fusion enzymes.
  • To discuss challenges and future perspectives in fusion enzyme technology.

Main Methods:

  • Literature review of fusion enzyme design strategies and applications.
  • Analysis of the impact of linker properties on fusion enzyme performance.
  • Discussion of case studies and recent research findings.

Main Results:

  • Fusion enzymes offer versatile solutions for industrial biocatalysis.
  • Linker properties significantly influence fusion enzyme function and stability.
  • Diverse applications demonstrate the potential of engineered fusion enzymes.

Conclusions:

  • Fusion enzyme technology is a powerful tool for developing novel biocatalysts.
  • Further research into linker optimization and application expansion is warranted.
  • Addressing current challenges will drive innovation in enzyme engineering for industry.