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Stability of fragrance patch test preparations applied in test chambers
M Mowitz1, E Zimerson, C Svedman
1Department of Occupational and Environmental Dermatology, Lund University, Skåne University Hospital, SE-20502 Malmö, Sweden. martin.mowitz@med.lu.se
The British Journal of Dermatology
|July 19, 2012
Summary
Fragrance allergens in petrolatum patch test preparations evaporate quickly from test chambers, potentially affecting results. Prepare tests just before use and store in a refrigerator for better stability.
Area of Science:
- Dermatology
- Allergen testing
- Analytical chemistry
Background:
- Petrolatum patch test preparations are often applied hours or days before patient testing.
- Fragrance compounds are volatile, raising concerns about the stability of these preparations over time.
Purpose of the Study:
- To assess the stability of petrolatum preparations containing fragrance mix (FM I) components stored in test chambers.
Main Methods:
- Liquid chromatography was used to analyze petrolatum preparations stored in test chambers.
- Storage conditions included room temperature and refrigeration for durations ranging from 4 to 144 hours.
Main Results:
- Four of seven fragrance allergens showed a ≥20% concentration decrease within 8 hours at room temperature.
- Refrigeration significantly improved stability, with only cinnamal showing a ≥20% decrease within 24 hours.
- Cinnamal and cinnamyl alcohol were more stable within the fragrance mix compared to individual preparations.
Conclusions:
- Fragrance allergens can evaporate from test chambers within hours, potentially impacting patch test outcomes.
- Preparing patch tests immediately before use and refrigerating them is recommended for improved accuracy.
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