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Related Experiment Videos

A comparative study on S-100 protein-immunoreactive cells in lymph nodes.

M Sugimura1, D Shirogane, Y Atoji

  • 1Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.

Nihon Juigaku Zasshi. the Japanese Journal of Veterinary Science
|October 1, 1990
PubMed
Summary

S-100 protein is found in various lymph node cells in mammals but not ducks. Its presence varies across species, with unique cells identified in guinea pigs and pigs.

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Area of Science:

  • Immunohistochemistry
  • Comparative immunology
  • Mammalian and avian lymph node biology

Background:

  • S-100 protein is a calcium-binding protein found in various tissues.
  • Its presence and function in lymph node cells across different species are not fully understood.
  • Lymph nodes are crucial for immune responses, involving diverse cell types.

Purpose of the Study:

  • To investigate the distribution of S-100 protein-immunoreactive cells in lymph nodes of diverse mammalian species and ducks.
  • To compare the expression patterns of S-100 protein and its subunits across species.
  • To identify species-specific variations in S-100 protein localization within lymph nodes.

Main Methods:

  • Immunohistochemical analysis of lymph node tissues from ducks and nine mammalian species.

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  • Detection of S-100 protein and its alpha and beta subunits using specific antibodies.
  • Microscopic examination to identify positive cells and their locations within lymph node compartments.

    • Main Results:

    • S-100 protein was detected in follicular dendritic cells (FDC), tangible-body macrophages (TBM), macrophages (MP), sinus endothelial cells (SEC), and interdigitating reticulum-like cells (IRC) in mammalian lymph nodes, but not in ducks (except neural elements).
    • S-100-positive FDC and TBM were consistently found in germinal centers of all mammals studied.
    • S-100 alpha subunit was present in FDC (except ducks, guinea pigs) and SEC (dog, cow, Japanese serow). Unique S-100 alpha-positive giant dendritic cells (GDC) were found in guinea pigs, and S-100-immunoreactive lymphocytes in pigs. S-100 beta subunit was found in arterial endothelial cells of pigs and cows.

    Conclusions:

    • S-100 protein expression in lymph node cells is a conserved feature in mammals but absent in ducks, suggesting species-specific roles.
    • Variations in S-100 protein and subunit distribution highlight distinct cellular compositions and functions across mammalian lymph nodes.
    • The identification of unique S-100-positive cells in specific species (guinea pig, pig) warrants further investigation into their immunological significance.