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Homogeneous assay for evaluation of aptamer-protein interaction
Gergely Lautner1, Zsófia Balogh, Anna Gyurkovics
1Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szt. Gellért tér 4, H-1111 Budapest, Hungary.
The Analyst
|July 19, 2012
Abstract:
We introduce Amplified Luminescent Proximity Homogenous Assay (ALPHA) to assess the K(D) value of aptamer-protein complexes as demonstrated through the study of apple stem pitting virus coat protein-specific aptamers. This method can be used as a simple, cost-effective method for screening aptamer-target protein interactions during aptamer selection.
