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qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping
Published on: May 25, 2015
PCR-based gene marker with a high efficiency genome scanning (HEGS) system: application for distinguishing among
Kazuhisa Terashima1, Chisato Funato, Teruyuki Matsumoto
1Tottori Mycological Institute, Tottori 689-1125, Japan. kin-ters@infosakyu.ne.jp
Abstract:
To establish a high throughput and cost-efficiency procedure for distinguishing among cultivars in Lentinula edodes, the polymorphism in the genes reported previously in this fungus was examined using PCR or PCR-RFLP techniques with a high-efficiency genome scanning (HEGS) system. As a result, PCR-based markers derived from eight genes (tyr, cap, ppa, IGS-RFLP, pri B-RFLP, mfbC-RFLP, gla-RFLP, xy-RFLP) showed polymorphisms among cultivars in this fungus and consequently, enabled to distinguish seventy-nine cultivars used in this study.
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