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Updated: May 20, 2026

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
Published on: September 23, 2021
Liquid chromatography and mass spectrometry of haem biosynthetic intermediates: a review
Christopher M Benton1, Chang Kee Lim
1Clinical Biochemistry, King's College Hospital, Denmark Hill, London, SE5 9RS, UK.
Abstract:
This article discusses the separation, analysis and characterisation of intermediates and oxidative by-products of the haem biosynthetic pathway by liquid chromatography and mass spectrometry. Techniques reviewed include high-performance liquid chromatography, ultra-high-performance liquid chromatography, capillary electrophoresis, ion mobility spectrometry, mass spectrometry and tandem mass spectrometry. The emphasis was on the analysis of biological and clinical samples.
