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Related Concept Videos

Induced-fit Model01:13

Induced-fit Model

Most chemical reactions in cells require enzymes—biological catalysts that speed up the reaction without being consumed or permanently changed. They reduce the activation energy needed to convert the reactants into products. Enzymes are proteins, that usually work by binding to a substrate—a reactant molecule that they act upon.
Enzymes exhibit substrate specificity, meaning that they can only bind to certain substrates. This is mainly determined by the shape and chemical characteristics of...
Enzymes02:34

Enzymes

Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions.
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
Introduction to Mechanisms of Enzyme Catalysis01:13

Introduction to Mechanisms of Enzyme Catalysis

For many years, scientists thought that enzyme-substrate binding took place in a simple "lock-and-key" fashion. This model stated that the enzyme and substrate fit together perfectly in one instantaneous step. However, current research supports a more refined view scientists call induced fit. The induced-fit model expands upon the lock-and-key model by describing a more dynamic interaction between enzyme and substrate. As the enzyme and substrate come together, their interaction causes a mild...
Introduction to Mechanisms of Enzyme Catalysis01:13

Introduction to Mechanisms of Enzyme Catalysis

For many years, scientists thought that enzyme-substrate binding took place in a simple "lock-and-key" fashion. This model stated that the enzyme and substrate fit together perfectly in one instantaneous step. However, current research supports a more refined view scientists call induced fit. The induced-fit model expands upon the lock-and-key model by describing a more dynamic interaction between enzyme and substrate. As the enzyme and substrate come together, their interaction causes a mild...
Introduction to Enzyme Kinetics01:19

Introduction to Enzyme Kinetics

Enzyme kinetics studies the rates of biochemical reactions. Scientists monitor the reaction rates for a particular enzymatic reaction at various substrate concentrations. Additional trials with inhibitors or other molecules that affect the reaction rate may also be performed.
The experimenter can then plot the initial reaction rate or velocity (Vo) of a given trial against the substrate concentration ([S]) to obtain a graph of the reaction properties. For many enzymatic reactions involving a...
Enzyme Kinetics01:19

Enzyme Kinetics

Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...

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Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects
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Published on: February 18, 2014

Development of the enzyme-substrate interactions concept inventory.

Stacey Lowery Bretz1, Kimberly J Linenberger

  • 1Department of Chemistry & Biochemistry, Miami University, Oxford, Ohio 45056, USA. bretzsl@muohio.edu.

Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology
|July 19, 2012
PubMed
Summary
This summary is machine-generated.

This study developed the Enzyme-Substrate Interactions Concept Inventory (ESICI) to assess student comprehension of enzyme-substrate interactions. The ESICI demonstrates reliability and validity for evaluating biochemistry student understanding.

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Area of Science:

  • Biochemistry Education
  • Enzyme Kinetics
  • Molecular Biology

Background:

  • Enzyme function is a core concept in biochemistry.
  • Student understanding of enzyme-substrate interactions is crucial for grasping biochemical processes.
  • Existing assessments may not adequately measure specific student knowledge in this area.

Purpose of the Study:

  • To develop and validate a reliable instrument to measure student understanding of enzyme-substrate interactions.
  • To provide a tool for assessing the effectiveness of biochemistry curricula.
  • To support research in biochemistry education.

Main Methods:

  • Development of a 15-item Enzyme-Substrate Interactions Concept Inventory (ESICI).
  • Establishment of validity and reliability through multiple assessment methods.
  • Administration of the ESICI to a large cohort of US biochemistry students (N=707).

Main Results:

  • The ESICI demonstrated strong psychometric properties, indicating good instrument quality.
  • Data from 707 students confirmed the reliability and validity of the ESICI.
  • Analysis provided insights into student understanding of enzyme-substrate interactions.

Conclusions:

  • The Enzyme-Substrate Interactions Concept Inventory (ESICI) is a validated tool for assessing student knowledge.
  • The ESICI can be utilized in both teaching and biochemistry education research.
  • Findings support the use of the ESICI to improve biochemistry instruction and learning outcomes.