Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Group 8 metallocenes as single-source precursors for the synthesis of light-element-stabilized FCC phases under extreme conditions.

Science and technology of advanced materials·2026
Same author

High-Pressure Synthesis of a Silicon-Rich Ruthenium Silicide, RuSi<sub>3</sub>, with a 9-Coordinated Ru Site and a Si-Si Dumbbell.

Inorganic chemistry·2026
Same author

A Translational Neural Network Mechanism of Resilience: Top-Down Control and Plasticity of the Visual Cortex Relates to Resilient Outcome and Performance.

Research (Washington, D.C.)·2026
Same author

Effects of the Uncoupling Protein 1 (UCP1) A-3826G Polymorphism on Taste Preferences in Healthy Young Japanese Adults.

Life (Basel, Switzerland)·2026
Same author

[A case of autoantibody-negative autoimmune encephalitis associated with mature ovarian teratoma, successfully treated with early ovariectomy].

Rinsho shinkeigaku = Clinical neurology·2026
Same author

An R83W mutation in Rab3A causes autosomal-dominant cerebellar ataxia.

Human molecular genetics·2026

Related Experiment Video

Updated: May 20, 2026

Real-time In Vivo Recording of Arabidopsis Calcium Signals During Insect Feeding Using a Fluorescent Biosensor
08:21

Real-time In Vivo Recording of Arabidopsis Calcium Signals During Insect Feeding Using a Fluorescent Biosensor

Published on: August 15, 2017

An improved genetically encoded red fluorescent Ca2+ indicator for detecting optically evoked action potentials.

Masamichi Ohkura1, Takuya Sasaki, Chiaki Kobayashi

  • 1Brain Science Institute, Saitama University, Saitama, Japan. mohkura@mail.saitama-u.ac.jp

Plos One
|July 19, 2012
PubMed
Summary
This summary is machine-generated.

Researchers developed R-CaMP1.07, an improved red fluorescent genetically encoded calcium indicator (GECI). This new GECI offers enhanced fluorescence response and reliably detects neuronal activity, aiding in neuroscience research.

More Related Videos

Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis
08:59

Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis

Published on: July 16, 2021

Imaging Ca2+ Dynamics in Cone Photoreceptor Axon Terminals of the Mouse Retina
09:05

Imaging Ca2+ Dynamics in Cone Photoreceptor Axon Terminals of the Mouse Retina

Published on: May 6, 2015

Related Experiment Videos

Last Updated: May 20, 2026

Real-time In Vivo Recording of Arabidopsis Calcium Signals During Insect Feeding Using a Fluorescent Biosensor
08:21

Real-time In Vivo Recording of Arabidopsis Calcium Signals During Insect Feeding Using a Fluorescent Biosensor

Published on: August 15, 2017

Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis
08:59

Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis

Published on: July 16, 2021

Imaging Ca2+ Dynamics in Cone Photoreceptor Axon Terminals of the Mouse Retina
09:05

Imaging Ca2+ Dynamics in Cone Photoreceptor Axon Terminals of the Mouse Retina

Published on: May 6, 2015

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Biophysics

Background:

  • Genetically encoded calcium indicators (GECIs) are essential for visualizing cellular activity.
  • Existing red fluorescent GECIs have limitations in sensitivity and response.
  • Precise monitoring of neuronal calcium transients is crucial for understanding brain function.

Purpose of the Study:

  • To develop and characterize an improved red fluorescent GECI with enhanced performance.
  • To evaluate the efficacy of the new GECI in detecting neuronal activity in real-time.
  • To assess the compatibility of the GECI with optogenetic tools.

Main Methods:

  • Directed mutagenesis of the R-GECO1 GECI to create R-CaMP1.07.
  • HeLa cell assays to quantify fluorescence response.
  • Electrophysiological recordings in hippocampal pyramidal neurons to assess neuronal activity.
  • Co-expression with channelrhodopsin-2 (ChR2) to evaluate optical stimulation compatibility.

Main Results:

  • R-CaMP1.07 demonstrated a 1.5-2 fold increase in fluorescence response compared to R-GECO1.
  • High sensitivity detection of single action potentials (APs) in neurons with >95% probability and SNR >7 at 50 Hz.
  • Calcium transient amplitudes linearly correlated with the number of APs.
  • No significant alteration of neuronal electrophysiological properties or synaptic activity.
  • R-CaMP1.07 is compatible with optogenetic stimulation using ChR2.

Conclusions:

  • R-CaMP1.07 represents a significant advancement in red fluorescent GECI technology.
  • This GECI enables sensitive and reliable monitoring of neuronal calcium dynamics.
  • R-CaMP1.07 facilitates simultaneous optical monitoring and manipulation of neuronal activity with single-cell resolution.