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Related Experiment Video

Updated: May 20, 2026

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
13:10

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Published on: June 9, 2017

Profiling cellular protein complexes by proximity ligation with dual tag microarray readout.

Maria Hammond1, Rachel Yuan Nong, Olle Ericsson

  • 1Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Rudbeck Laboratory, Uppsala University, Uppsala, Sweden.

Plos One
|July 19, 2012
PubMed
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We developed a new method to measure all protein interactions in biological samples. This technique combines proximity ligation assay with dual tag microarrays for scalable analysis in research and diagnostics.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Proteomics

Background:

  • Protein-protein interactions are crucial for understanding biological processes.
  • Analyzing these interactions is vital for drug discovery and disease diagnostics.

Purpose of the Study:

  • To establish a scalable method for comparing pairwise protein interactions between biological samples.
  • To enable comprehensive analysis of protein interaction networks.

Main Methods:

  • Utilized a proximity ligation assay (PLA) combined with dual tag microarrays.
  • Protein identities were encoded using DNA oligonucleotides attached to antibodies.
  • Reporter DNA molecules with sample barcodes were generated through antibody-mediated proximity and ligation.

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Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay
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Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay

Published on: October 20, 2018

Related Experiment Videos

Last Updated: May 20, 2026

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
13:10

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Published on: June 9, 2017

In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells
08:58

In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells

Published on: September 2, 2019

Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay
09:18

Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay

Published on: October 20, 2018

Main Results:

  • Successfully detected pairwise interactions among five proteins in cellular lysates and fixed cells.
  • Demonstrated the technique's ability to encode protein identity and sample origin in reporter DNA.
  • Validated the dual tag microarray readout for decoding interaction information.

Conclusions:

  • The developed technique offers a general strategy for analyzing pairwise interactions in large molecular sets.
  • This method provides a scalable and robust approach for protein interaction analysis.
  • The findings have implications for advancing basic biology research, drug development, and diagnostics.