Related Experiment Video
Updated: May 20, 2026
09:24
Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy
Published on: January 30, 2020
Image formation by linear and nonlinear digital scanned light-sheet fluorescence microscopy with Gaussian and Bessel
Biomedical Optics Express
|July 19, 2012
Abstract:
We present the implementation of a combined digital scanned light-sheet microscope (DSLM) able to work in the linear and nonlinear regimes under either Gaussian or Bessel beam excitation schemes. A complete characterization of the setup is performed and a comparison of the performance of each DSLM imaging modality is presented using in vivoCaenorhabditis elegans samples. We found that the use of Bessel beam nonlinear excitation results in better image contrast over a wider field of view.
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