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Updated: May 20, 2026

Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites
Published on: November 9, 2014
DNA-damaging effects of dental bleaching agents
K L Pligina1, I A Rodina, T V Shevchenko
1Testing Laboratory of Biological Activity and Toxicological Safety, Moscow Factory of Ecological Engineering and Ecological Nutrition DIOD, Russia. kirapligina@yandex.ru
Abstract:
We studied DNA-damaging effects of dental bleaching systems containing hydrogen peroxide and/or carbamide peroxide by the "comet assay" (alkaline version). Dental bleaching systems in a hydrogen peroxide concentration range from 0.03 to 30 mM produced a genotoxic effect on isolated HeLa cells in vitro comparable with the effects of pharmacopoeial hydrogen peroxide or urea peroxide. Catalase protected the cells against products containing hydrogen peroxide and had no effect on the genotoxicity of samples containing carbamide peroxide.
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