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Updated: May 20, 2026

Single Cell Electroporation in vivo within the Intact Developing Brain

Single Cell Electroporation in vivo within the Intact Developing Brain

Published on: July 11, 2008

Single-cell electroporation using a multifunctional pipette.

Alar Ainla¹, Shijun Xu, Nicolas Sanchez

¹Department of Chemical and Biological Engineering, Chalmers University of Technology, S-41296 Göteborg, Sweden.

|July 20, 2012

Summary

This summary is machine-generated.

This study introduces a new tool for single-cell analysis, combining electroporation and analyte delivery in one pipette. This novel platform enables precise manipulation of individual cells within their culture environment.

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Published on: October 6, 2020

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Last Updated: May 20, 2026

Single Cell Electroporation in vivo within the Intact Developing Brain

Single Cell Electroporation in vivo within the Intact Developing Brain

Published on: July 11, 2008

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery

Published on: August 7, 2014

Single-Cell Electroporation across Different Organotypic Slice Culture of Mouse Hippocampal Excitatory and Class-Specific Inhibitory Neurons

Single-Cell Electroporation across Different Organotypic Slice Culture of Mouse Hippocampal Excitatory and Class-Specific Inhibitory Neurons

Published on: October 6, 2020

Area of Science:

Biotechnology
Cell Biology
Microfluidics

Background:

Precise manipulation of single cells is crucial for various biological studies.
Existing methods often require complex setups or external manipulation tools.

Purpose of the Study:

To develop a novel multifunctional pipette for simultaneous single-cell electroporation and local analyte delivery.
To integrate low-resistance metallic electrodes into a polydimethylsiloxane (PDMS) pipette.
To characterize the performance of this integrated system.

Main Methods:

Fabrication of a polydimethylsiloxane (PDMS) pipette with integrated low-resistance metallic electrodes.
Characterization of electrode performance, including resistance and functionality.
Demonstration of simultaneous single-cell electroporation and local analyte delivery within the cell culture environment.
Comparative analysis against external carbon microelectrodes.

Main Results:

Successful fabrication of a multifunctional pipette with integrated metallic electrodes.
Demonstrated capability for precise, localized electroporation and analyte delivery to single cells.
Characterization of the system's effectiveness, benefits, and limitations.
Comparison highlighting advantages over external microelectrode systems.

Conclusions:

The novel multifunctional pipette offers a streamlined approach for advanced single-cell analysis.
This integrated platform enhances precision and efficiency in cellular manipulation and delivery.
The developed system shows significant potential for applications in cell biology research and drug delivery studies.