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Updated: May 20, 2026
In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography
Published on: July 24, 2020
Comparison of retinal nerve fiber layer thickness measurements by spectral-domain optical coherence tomography
Roy de Kinkelder1, Daniel M de Bruin, Frank D Verbraak
1Department of Biomedical Engineering & Physics, Academic Medical Center, University of Amsterdam, PO Box 22700, NL-1100 DE Amsterdam, The Netherlands. r.dekinkelder@amc.nl
Abstract:
To quantify differences in nerve fiber layer thickness measurements by various spectral-domain optical coherence tomography (SD-OCT) systems, we developed a phantom eye model. We tested twelve SD-OCT systems of four manufacturers. All systems combined overestimated the 49 µm thick phantom RNFL thickness on average by 18 µm. Within brands, thickness measurements differed statistically significant for one Topcon, one RTVue and one Cirrus. Between brands, thickness determined with RTVue and Topcon differed statistically significant from Cirrus and Spectralis. The maximum difference between mean thicknesses is 3.6 µm within brands and 7.7 µm between brands.
