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Related Experiment Video

Updated: May 20, 2026

Production of Autologous Platelet-Rich Plasma for Boosting In Vitro Human Fibroblast Expansion
08:34

Production of Autologous Platelet-Rich Plasma for Boosting In Vitro Human Fibroblast Expansion

Published on: February 24, 2021

A scalable, micropore, platelet rich plasma separation device.

Mary Nora Dickson1, Levy Amar, Michael Hill

  • 1Chemical Engineering, Columbia University in the City of New York, 801 Mudd, 500 W 120th St., New York, NY, 10027, USA. marydickson@gmail.com

Biomedical Microdevices
|July 20, 2012
PubMed
Summary

A new low-energy platelet-rich-plasma (PRP) separator efficiently extracts platelets for emergency use. This device achieves high platelet recovery rates with minimal red blood cell contamination.

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Area of Science:

  • Biomedical Engineering
  • Hematology
  • Medical Device Design

Background:

  • Current methods for platelet separation may not be suitable for rapid, on-site applications, particularly in military or emergency settings.
  • The need for efficient and scalable platelet-rich plasma (PRP) separation technology is critical for transfusion medicine and point-of-care treatments.

Purpose of the Study:

  • To design and validate a novel, low-energy platelet-rich-plasma (PRP) separator for rapid production of platelet concentrate.
  • To assess the efficiency of platelet extraction and minimize erythrocyte contamination for military and emergency applications.

Main Methods:

  • Developed a microfiltration system using two sieve stages with varying pore sizes to separate platelets from erythrocytes.
  • Employed a multi-pass system with platelet-free plasma return to enhance overall platelet extraction efficiency.

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Preparation of Pooled Human Platelet Lysate (pHPL) as an Efficient Supplement for Animal Serum-Free Human Stem Cell Cultures
08:32

Preparation of Pooled Human Platelet Lysate (pHPL) as an Efficient Supplement for Animal Serum-Free Human Stem Cell Cultures

Published on: October 30, 2009

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Last Updated: May 20, 2026

Production of Autologous Platelet-Rich Plasma for Boosting In Vitro Human Fibroblast Expansion
08:34

Production of Autologous Platelet-Rich Plasma for Boosting In Vitro Human Fibroblast Expansion

Published on: February 24, 2021

Preparation of Pooled Human Platelet Lysate (pHPL) as an Efficient Supplement for Animal Serum-Free Human Stem Cell Cultures
08:32

Preparation of Pooled Human Platelet Lysate (pHPL) as an Efficient Supplement for Animal Serum-Free Human Stem Cell Cultures

Published on: October 30, 2009

  • Tested prototype microsieves (3 mm x 3 mm) at different filtrate flow rates (20-100 µL/min) and pore sizes (1.2-3.5 µm).

    • Main Results:

    • Achieved high platelet passage ratios (up to 150%) using 3.5 µm filters at a flow rate of 100 µL/min.
    • Demonstrated erythrocyte leakage consistently below 2,000/µL, meeting stringent AABB standards.
    • Calculated that a 13.7 cm² filter area is sufficient to produce 50 mL of platelet concentrate within 30 minutes.

    Conclusions:

    • The designed low-energy PRP separator is effective and scalable for producing platelet concentrate rapidly.
    • This technology offers a promising solution for transfusion support in resource-limited and emergency environments.
    • The system's ability to achieve high yields with minimal contamination addresses critical needs in transfusion medicine.