Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Asymmetric Lipid Bilayer01:35

Asymmetric Lipid Bilayer

Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The role of lipid oxidation on electrical properties of planar lipid bilayers and its importance for understanding electroporation.

Bioelectrochemistry (Amsterdam, Netherlands)·2023
Same author

The good and the bad of cell membrane electroporation.

Acta chimica Slovenica·2021
Same author

Effect of the cholesterol on electroporation of planar lipid bilayer.

Bioelectrochemistry (Amsterdam, Netherlands)·2021
Same author

Calcium ion effect on phospholipid bilayers as cell membrane analogues.

Bioelectrochemistry (Amsterdam, Netherlands)·2021
Same author

Water Pores in Planar Lipid Bilayers at Fast and Slow Rise of Transmembrane Voltage.

Membranes·2021
Same author

Internal configuration and electric potential in planar negatively charged lipid head group region in contact with ionic solution.

Bioelectrochemistry (Amsterdam, Netherlands)·2016

Related Experiment Video

Updated: May 20, 2026

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
20:00

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Published on: October 31, 2015

System for measuring planar lipid bilayer properties.

Andraž Polak1, Boštjan Mulej, Peter Kramar

  • 1Faculty of Electrical Engineering, University of Ljubljana, Tržaška 25, 1000 Ljubljana, Slovenia. andraz.polak@fe.uni-lj.si

The Journal of Membrane Biology
|July 20, 2012
PubMed
Summary
This summary is machine-generated.

We developed an automated system to measure planar lipid bilayer properties, including capacitance and breakdown voltage. This system utilizes a folding method for bilayer formation and precise temperature control for accurate electrical measurements.

More Related Videos

A Nanobar-Supported Lipid Bilayer System for the Study of Membrane Curvature Sensing Proteins in vitro
08:27

A Nanobar-Supported Lipid Bilayer System for the Study of Membrane Curvature Sensing Proteins in vitro

Published on: November 30, 2022

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer
10:34

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Published on: April 23, 2017

Related Experiment Videos

Last Updated: May 20, 2026

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
20:00

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Published on: October 31, 2015

A Nanobar-Supported Lipid Bilayer System for the Study of Membrane Curvature Sensing Proteins in vitro
08:27

A Nanobar-Supported Lipid Bilayer System for the Study of Membrane Curvature Sensing Proteins in vitro

Published on: November 30, 2022

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer
10:34

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Published on: April 23, 2017

Area of Science:

  • Biophysics
  • Materials Science
  • Electrical Engineering

Background:

  • Planar lipid bilayers are crucial models for cell membranes.
  • Accurate measurement of their electrical properties is essential for understanding membrane function and developing new technologies.

Purpose of the Study:

  • To present an automated system for measuring planar lipid bilayer properties.
  • To enable precise control over experimental conditions, such as temperature and applied electrical signals.

Main Methods:

  • Automated formation of planar lipid bilayers using a folding method.
  • Integration of an LCR meter, temperature-regulated bath, and a four-electrode measurement chamber.
  • Application of various voltage- or current-clamp signals via a control unit and actuators.

Main Results:

  • The system successfully measures capacitance and breakdown voltage of planar lipid bilayers.
  • Temperature regulation is achievable between 15 and 55 °C.
  • The system exhibits cutoff frequencies of 11 kHz (voltage-clamp) and 17 kHz (current-clamp).

Conclusions:

  • The developed system provides a robust and automated platform for characterizing planar lipid bilayer electrical properties.
  • This facilitates advanced research in membrane biophysics and related fields.
  • The system's automation and precise control enhance reproducibility and efficiency in bilayer measurements.