Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Feedback Regulation of Calcium Concentration01:27

Feedback Regulation of Calcium Concentration

Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
The Role of Ion Channels in Neuronal Computation01:19

The Role of Ion Channels in Neuronal Computation

A postsynaptic neuron usually receives numerous impulses from several other presynaptic neurons. The axon hillock of the postsynaptic neuron integrates all these signals and determines the likelihood of firing an action potential.
Sometimes a single EPSP is strong enough to induce an action potential in the postsynaptic neuron. However, multiple presynaptic inputs must often create EPSPs around the same time for the postsynaptic neuron to be sufficiently depolarized to fire an action potential.
Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
Amplifying Signals via Second Messengers01:15

Amplifying Signals via Second Messengers

Many receptor binding ligands are hydrophilic; they do not cross the cell membrane but bind to cell-surface receptors. Thus, their message must be relayed by second messengers present in the cell cytoplasm. There are several second messenger pathways, each with its own way of relaying information. For example, the G protein-coupled receptors can activate both phosphoinositol and cyclic AMP (cAMP) second messenger pathways. The phosphoinositol pathway is active when the receptor induces...
Voltage-gated Ion Channels01:26

Voltage-gated Ion Channels

Voltage-gated ion channels are transmembrane proteins that open and close in response to changes in the membrane potential. They are present on the membranes of all electrically excitable cells such as neurons, heart, and muscle cells.
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...
Mechanically-gated Ion Channels01:12

Mechanically-gated Ion Channels

Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Astrocyte-Targeted connexin43 Hemichannel Inhibition Prevents Radiation-Induced Energy Transporter Decrease in Neurons and Astrocytic proBDNF Transport to Synapses.

Glia·2025
Same author

From IP3RPEP6 Inhibition of IP<sub>3</sub> receptor channels to insights: do channel subunits collaborate or cooperate?

Cellular and molecular life sciences : CMLS·2025
Same author

Myelin sheaths can act as compact temporary oxygen storage units as modeled by an electrical RC circuit model.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

High rate of detected variants in male <i>PLCZ1</i> and <i>ACTL7A</i> genes causing failed fertilization after ICSI.

Human reproduction open·2024
Same author

Fundamental Patterns of Structural Evolution Revealed by Chromosome-Length Genomes of Cactophilic Drosophila.

Genome biology and evolution·2024
Same author

Integrating and optimizing tonabersat in standard glioblastoma therapy: A preclinical study.

PloS one·2024

Related Experiment Video

Updated: May 20, 2026

Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ
09:34

Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ

Published on: January 7, 2019

Intercellular Ca(2+) waves: mechanisms and function.

Luc Leybaert1, Michael J Sanderson

  • 1Department of Basic Medical Sciences, Physiology Group, Faculty of Medicine & Health Sciences, Ghent University, Ghent, Belgium. Luc.Leybaert@UGent.be

Physiological Reviews
|July 20, 2012
PubMed
Summary

Intercellular calcium (Ca2+) waves coordinate cell responses via internal or external messengers. These waves are crucial in both healthy tissues and diseases across various organs.

More Related Videos

Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells
10:46

Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells

Published on: July 16, 2013

Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators
11:33

Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators

Published on: March 22, 2019

Related Experiment Videos

Last Updated: May 20, 2026

Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ
09:34

Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ

Published on: January 7, 2019

Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells
10:46

Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells

Published on: July 16, 2013

Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators
11:33

Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators

Published on: March 22, 2019

Area of Science:

  • Cellular Biology
  • Physiology
  • Biochemistry

Background:

  • Intercellular calcium (Ca2+) waves (ICWs) are fundamental for coordinating multicellular responses.
  • ICWs involve synchronized increases in intracellular Ca2+ propagation through cell syncytia.
  • While studied in vitro, recent research emphasizes ICWs in vivo.

Purpose of the Study:

  • To review the mechanisms of ICW initiation and propagation.
  • To identify key intra- and extracellular messengers involved in ICWs.
  • To discuss the proposed physiological functions of ICWs.

Main Methods:

  • Literature review of studies on intercellular calcium waves.
  • Analysis of mechanisms involving gap junctions and paracrine signaling.
  • Identification of key signaling molecules like inositol 1,4,5-trisphosphate and ATP.

Main Results:

  • ICWs are triggered by diverse stimuli, involving internal Ca2+ store release.
  • Propagation relies on gap junctions or paracrine signaling via extracellular messengers.
  • ICWs are implicated in normal physiology and pathophysiology in organs like the brain and liver.

Conclusions:

  • ICWs are a vital mechanism for intercellular communication and multicellular coordination.
  • Understanding ICW messengers (IP3 and ATP) and pathways is key to their function.
  • ICWs play significant roles in diverse physiological and pathophysiological processes.