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Published on: May 22, 2020
Spatial and temporal measurements of temperature and cell viability in response to nanoparticle-mediated photothermal
Jon R Whitney1, Amanda Rodgers, Erica Harvie
1Department of Mechanical Engineering & School of Biomedical Engineering & Sciences, Virginia Tech, VA 24061, USA. jonwhit@vt.edu
Aim:
Nanoparticle-enhanced photothermal therapy is a promising alternative to tumor resection. However, quantitative measurements of cellular response to these treatments are limited. This article introduces a Bimodal Enhanced Analysis of Spatiotemporal Temperature (BEAST) algorithm to rapidly determine the viability of cancer cells in vitro following photothermal therapy alone or in combination with nanoparticles.
Materials & Methods:
To illustrate the capability of the BEAST viability algorithm, single wall carbon nanohorns were added to renal cancer (RENCA) cells in vitro and time-dependent spatial temperature maps measured with an infrared camera during laser therapy were correlated with post-treatment cell viability distribution maps obtained by cell-staining fluorescent microscopy.
Conclusion:
The BEAST viability algorithm accurately and rapidly determined the cell viability as a function of time, space and temperature.
