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Related Experiment Video

Updated: May 20, 2026

Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis
07:28

Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis

Published on: July 23, 2015

Comprehensive proteomic analysis of human dentin.

Michal Jágr1, Adam Eckhardt, Statis Pataridis

  • 1Institute of Physiology, Academy of Sciences of the Czech Republic vvi, Prague, Czech Republic. jagr@biomed.cas.cz

European Journal of Oral Sciences
|July 21, 2012
PubMed
Summary

This study mapped the human dentin proteome using advanced mass spectrometry, identifying 289 proteins, including 90 novel ones and nine found in vivo for the first time.

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Area of Science:

  • Biochemistry
  • Proteomics
  • Dentistry

Background:

  • Proteomic analysis is crucial for understanding biological systems.
  • Human dentin's complex protein composition remains incompletely characterized.

Purpose of the Study:

  • To comprehensively investigate the proteomic profile of human dentin.
  • To identify novel proteins and their functions within dentin.

Main Methods:

  • Proteins extracted from human molars.
  • Analyzed using SDS-PAGE, 2D-PAGE, and liquid chromatography/high-resolution tandem mass spectrometry.
  • Proteins digested with trypsin for peptide analysis.

Main Results:

  • Identified 289 high-confidence proteins in human dentin.

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Sampling Human Indigenous Saliva Peptidome Using a Lollipop-Like Ultrafiltration Probe: Simplify and Enhance Peptide Detection for Clinical Mass Spectrometry

Published on: August 7, 2012

Accessing the Cytotoxicity and Cell Response to Biomaterials
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Accessing the Cytotoxicity and Cell Response to Biomaterials

Published on: July 8, 2021

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Last Updated: May 20, 2026

Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis
07:28

Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis

Published on: July 23, 2015

Sampling Human Indigenous Saliva Peptidome Using a Lollipop-Like Ultrafiltration Probe: Simplify and Enhance Peptide Detection for Clinical Mass Spectrometry
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Sampling Human Indigenous Saliva Peptidome Using a Lollipop-Like Ultrafiltration Probe: Simplify and Enhance Peptide Detection for Clinical Mass Spectrometry

Published on: August 7, 2012

Accessing the Cytotoxicity and Cell Response to Biomaterials
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Accessing the Cytotoxicity and Cell Response to Biomaterials

Published on: July 8, 2021

  • Discovered 90 proteins previously undetected in dentin.
  • Reported nine hypothetical proteins identified in a human sample for the first time.
  • Identified proteins involved in calcium binding, extracellular matrix, cytoskeleton, immune response, and transport.

    • Conclusions:

    • This study presents the first proteomic analysis of human dentin utilizing two-dimensional electrophoresis.
    • The findings expand the known human dentin proteome and reveal novel protein functions.
    • This research provides a foundation for future studies on dentin biology and pathology.