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Related Concept Videos

The Blood-brain Barrier00:49

The Blood-brain Barrier

Overview
Physiological Barriers01:25

Physiological Barriers

Physiological barriers are semi-permeable cellular structures restricting drug diffusion into intracellular compartments and tissues. There are six types of physiological barriers: blood endothelial, cell membrane, blood-brain, blood-cerebrospinal fluid (CSF), blood-placenta, and blood-testis barriers.
The blood endothelial barrier is the most porous of these. It allows all small ionized, un-ionized, and lipophilic molecules to pass through the endothelial lining into the interstitial space...

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Related Experiment Video

Updated: May 20, 2026

Isolation of Cerebral Capillaries from Fresh Human Brain Tissue
06:35

Isolation of Cerebral Capillaries from Fresh Human Brain Tissue

Published on: September 12, 2018

Blood-brain barrier unlocked.

N Goldstein1, R Goldstein, D Terterov

  • 1Department of Human and Animal Physiology, Biological Faculty, Lomonosov Moscow State University, 119991 Moscow, Russia. dr.naum.goldstein@googlemail.com

Biochemistry. Biokhimiia
|July 21, 2012
PubMed
Summary
This summary is machine-generated.

Hydrogen peroxide (H2O2) enables rapid delivery of dopamine into the brain via nasal application. This method significantly increases dopamine levels in key brain regions and restores motor activity, offering a new therapeutic strategy for neurological diseases.

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Rat Model of Blood-brain Barrier Disruption to Allow Targeted Neurovascular Therapeutics
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Rat Model of Blood-brain Barrier Disruption to Allow Targeted Neurovascular Therapeutics

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Reconstruction of the Blood-Brain Barrier In Vitro to Model and Therapeutically Target Neurological Disease
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Reconstruction of the Blood-Brain Barrier In Vitro to Model and Therapeutically Target Neurological Disease

Published on: October 20, 2023

Related Experiment Videos

Last Updated: May 20, 2026

Isolation of Cerebral Capillaries from Fresh Human Brain Tissue
06:35

Isolation of Cerebral Capillaries from Fresh Human Brain Tissue

Published on: September 12, 2018

Rat Model of Blood-brain Barrier Disruption to Allow Targeted Neurovascular Therapeutics
08:43

Rat Model of Blood-brain Barrier Disruption to Allow Targeted Neurovascular Therapeutics

Published on: November 30, 2012

Reconstruction of the Blood-Brain Barrier In Vitro to Model and Therapeutically Target Neurological Disease
06:19

Reconstruction of the Blood-Brain Barrier In Vitro to Model and Therapeutically Target Neurological Disease

Published on: October 20, 2023

Area of Science:

  • Neuroscience
  • Pharmacology
  • Biochemistry

Background:

  • The blood-brain barrier (BBB) restricts the entry of many neuroactive drugs into the brain.
  • Efficient methods for enhancing BBB permeability for drug delivery are needed.
  • Dopamine, a crucial neurotransmitter, normally does not penetrate the BBB.

Purpose of the Study:

  • To investigate the delivery of dopamine into the brain using intranasal administration combined with hydrogen peroxide (H2O2).
  • To evaluate the effect of this delivery method on dopamine levels in specific brain regions and on motor activity in a rat model.

Main Methods:

  • Intranasal co-administration of (3)H-labeled dopamine and H2O2 in rats.
  • Measurement of dopamine levels in hypothalamus and striatum.
  • Assessment of motor activity in a haloperidol-induced catalepsy model.

Main Results:

  • Intranasal dopamine + H2O2 significantly increased dopamine levels in the hypothalamus (45-fold) and striatum (30-fold) within minutes.
  • Motor activity was restored in rats with haloperidol-induced catalepsy 90 seconds after intranasal dopamine + H2O2 administration.
  • No significant effects were observed when H2O2 was replaced with saline.

Conclusions:

  • Simultaneous intranasal administration of dopamine and H2O2 facilitates rapid and efficient drug delivery into the brain.
  • This approach demonstrates the potential of exogenous reactive oxygen species (exoROS) in modulating BBB permeability.
  • The findings suggest a promising new therapeutic strategy for neurological disorders requiring enhanced brain drug delivery.