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Related Experiment Video

Updated: May 20, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

A novel quantitative proteomics workflow by isobaric terminal labeling.

Shu-Jun Yang1, Ai-Ying Nie, Lei Zhang

  • 1Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China.

Journal of Proteomics
|July 21, 2012
PubMed
Summary

We developed a novel MS2 quantification approach, quantitation by isobaric terminal labeling (QITL), using (18)O labeling and dimethylation. This method accurately quantifies peptides in complex samples like liver tissue for proteomic research.

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Area of Science:

  • Proteomics
  • Mass Spectrometry
  • Biochemistry

Background:

  • Quantitative proteomics relies on accurate peptide measurement.
  • Existing methods for isobaric labeling in MS2 spectra offer accurate quantification.
  • Novel approaches are needed to enhance accuracy and applicability.

Purpose of the Study:

  • To develop and validate a novel MS2 quantification approach named quantitation by isobaric terminal labeling (QITL).
  • To couple (18)O labeling with dimethylation for peptide quantification.
  • To assess QITL's applicability in complex biological samples and disease profiling.

Main Methods:

  • Developed QITL by combining C-terminal (18)O labeling with N-terminal dimethylation.
  • Utilized trypsin-digested peptides labeled with (16)O or (18)O and formaldehyde-d(2) or formaldehyde.

More Related Videos

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
14:51

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

Related Experiment Videos

Last Updated: May 20, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
14:51

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

  • Generated isobaric-labeled peptides for MS2 quantification using b, y fragment ion pairs.

    • Main Results:

    • Demonstrated QITL feasibility using standard proteins, achieving accurate and reproducible quantification over a wide dynamic range.
    • Verified QITL's applicability for large-scale quantitative analysis in complex rat liver samples.
    • Applied QITL to profile the quantitative proteome of hepatocellular carcinoma (HCC) and adjacent non-tumor tissues.

    Conclusions:

    • QITL is a simple, low-cost, and accurate method for quantitative proteomic analysis.
    • QITL can be widely applied to various biological samples, including cell lines, tissues, and body fluids.
    • QITL facilitates quantitative proteomic research, particularly in disease profiling like HCC.