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Inhibition of voltage-gated K+ channels and Ca2+ channels by diphenidol
Yuk-Man Leung1, Kar-Lok Wong, Ka-Shun Cheng
1Graduate Institute of Neural and Cognitive Sciences, China Medical University, Taichung 40402, Taiwan. thcheng@mail.cmu.edu.tw
Background:
Although diphenidol has long been deployed as an anti-emetic and anti-vertigo drug, its mechanism of action remains unclear. In particular, little is known as to how diphenidol affects neuronal ion channels. Recently, we showed that diphenidol blocked neuronal voltage-gated Na(+) channels, causing spinal blockade of motor function, proprioception and nociception in rats. In this work, we investigated whether diphenidol could also affect voltage-gated K(+) and Ca(2+) channels.
Methods:
Electrophysiological experiments were performed to study ion channel activities in two neuronal cell lines, namely, neuroblastoma N2A cells and differentiated NG108-15 cells.
Results:
Diphenidol inhibited voltage-gated K(+) channels and Ca(2+) channels, but did not affect store-operated Ca(2+) channels.
Conclusion:
Diphenidol is a non-specific inhibitor of voltage-gated ion channels in neuronal cells.
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