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Related Experiment Video

Updated: May 20, 2026

Imaging Features of Systemic Sclerosis-Associated Interstitial Lung Disease
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Imaging Features of Systemic Sclerosis-Associated Interstitial Lung Disease

Published on: June 16, 2020

Red blood cell alterations in systemic sclerosis: a pilot study.

Antonello Giovannetti1, Lucrezia Gambardella, Donatella Pietraforte

  • 1Department of Clinical Medicine, Clinical Immunology Unit-Scleroderma Center, Sapienza University of Rome, Rome, Italy.

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
|July 21, 2012
PubMed
Summary

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Systemic oxidative imbalance in systemic sclerosis alters red blood cell integrity. Red blood cells show reduced thiols and altered proteins in limited cutaneous sclerosis, and increased oxidative stress in diffuse cutaneous sclerosis.

Area of Science:

  • Hematology
  • Immunology
  • Rheumatology

Background:

  • Systemic sclerosis is characterized by systemic oxidative imbalance.
  • Red blood cell integrity may be affected by oxidative stress.

Purpose of the Study:

  • To investigate the impact of systemic oxidative imbalance on red blood cell integrity in systemic sclerosis patients.
  • To analyze red blood cell aging markers in relation to disease subsets.

Main Methods:

  • Erythrocytes from 39 systemic sclerosis patients and 30 healthy donors were analyzed.
  • Flow and static cytometry were used to measure reactive oxygen species, total thiols, and red blood cell aging markers (glycophorin A, band 3, CD47, phosphatidylserine externalization).
  • Analyses considered diffuse cutaneous sclerosis and limited cutaneous sclerosis subsets.

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Published on: June 16, 2020

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10:07

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Published on: March 17, 2023

Main Results:

  • Limited cutaneous sclerosis patients showed reduced intracellular thiols and loss of glycophorin A, band 3, and CD47.
  • Diffuse cutaneous sclerosis patients exhibited increased reactive oxygen species and CD47 expression.
  • Increased phosphatidylserine externalization was observed in both subsets, correlating with disease severity and nailfold capillaroscopy findings.

Conclusions:

  • Red blood cells exhibit altered integrity in systemic sclerosis, varying between disease subsets.
  • Red blood cells show potential as biomarkers for clinical management of systemic sclerosis.