Related Experiment Video
Updated: May 20, 2026
Intratracheal Instillation of Stem Cells in Term Neonatal Rats
Published on: May 4, 2020
Cell therapy with adipose tissue-derived stem/stromal cells for elastase-induced pulmonary emphysema in rats
Naoki Furuya1, Mitsuko Takenaga, Yuki Ohta
1Division of Respiratory & Infectious Diseases, Department of Internal Medicine, St Marianna University School of Medicine, Kawasaki, Japan.
Aims:
The purpose of this study was to elucidate the mechanism underlying the effects of adipose tissue-derived stem/stromal cell (ASC) transplantation on porcine pancreatic elastase-induced emphysema.
Materials & Methods:
ASCs (2.5 × 10(6)) were transplanted into pancreatic elastase (250 U/kg)-treated rats, after which gas exchange and growth factor/cytokine levels in lung tissue were determined.
Results:
ASC transplantation restored pulmonary function (arterial oxygen tension and alveolar-arterial oxygen tension difference) almost to that of normal animals. Enlargement of the alveolar airspaces was inhibited. HGF and CINC-1 levels were significantly higher in the ASC group even at 2 weeks after transplantation. Sponge implantation with CINC-1 induced neovascular formation with increased HGF. In vitro secretion of HGF and CINC-1 from ASCs was promoted in the presence of IL-1β.
Conclusion:
Not only HGF, but also CINC-1, secreted from transplanted and viable ASCs presumably contributed to lung repair through angiogenesis.
