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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

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Related Experiment Video

Updated: May 20, 2026

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

How does Tra2β protein regulate tissue-specific RNA splicing?

David J Elliott1, Andrew Best, Caroline Dalgliesh

  • 1Institute of Genetic Medicine, Newcastle University, Central Parkway, Newcastle upon Tyne, UK. David.Elliott@ncl.ac.uk

Biochemical Society Transactions
|July 24, 2012
PubMed
Summary
This summary is machine-generated.

The splicing regulator Tra2β binds AGAA-rich sequences on exons, influencing tissue-specific alternative splicing. This protein is crucial for mouse development and its targets are key to understanding gene regulation.

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Last Updated: May 20, 2026

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Area of Science:

  • Molecular Biology
  • Genetics
  • RNA Biology

Background:

  • Tra2β is a conserved splicing regulator essential for mouse development.
  • Recent studies identified Tra2β's physiological RNA targets, revealing its molecular mechanisms.
  • Tra2β binds AGAA-rich sequences predominantly on exons across the transcriptome.

Purpose of the Study:

  • To review the properties of Tra2β.
  • To examine Tra2β-regulated target exons.
  • To elucidate mechanisms by which Tra2β drives tissue-specific splicing patterns.

Main Methods:

  • Transcriptome-wide analysis of Tra2β binding sites.
  • In vitro binding assays for Tra2β and target exons.
  • In cellulo splicing assays following Tra2β co-expression and depletion.

Main Results:

  • Tra2β strongly binds to specific exons with high concentrations of binding sites.
  • Co-expression of Tra2β activates splicing inclusion of these target exons.
  • Genetic depletion of Tra2β significantly down-regulates splicing inclusion of target exons.

Conclusions:

  • Tra2β plays a critical role in regulating alternative splicing, particularly for tissue-specific exons.
  • Despite even expression, Tra2β can drive distinct tissue-specific splicing outcomes.
  • Understanding Tra2β's mechanisms provides insights into gene regulation and development.