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Updated: May 20, 2026

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
11:00

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

Terminal loop-mediated control of microRNA biogenesis

Nila Roy Choudhury1, Gracjan Michlewski

  • 1Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, UK.

Biochemical Society Transactions
|July 24, 2012
PubMed

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MicroRNAs

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
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Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
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