Related Experiment Video
Updated: May 7, 2026
11:32
Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection
Published on: December 4, 2010
Transcription, splicing, and release: are we there yet?
Ranjan Sen1, Sebastian D Fugmann
1Laboratory of Molecular Biology and Immunology, National Institute on Aging, 251 Bayview Boulevard, Baltimore, MD 21224, USA. ranjan.sen@nih.gov
Cell
|July 24, 2012
Summary
Inflammatory responses in macrophages are controlled by transcription initiation, according to Bhatt et al. Full-length RNAs with introns accumulate on chromatin for processing before becoming functional mRNA.
Area of Science:
- Molecular Biology
- Immunology
- Gene Regulation
Background:
- Lipopolysaccharide (LPS) is a potent activator of inflammatory responses in macrophages.
- Gene expression regulation is crucial for understanding cellular responses to stimuli like LPS.
- Previous studies suggested post-transcriptional regulation plays a significant role in inflammatory gene expression.
Discussion:
- Bhatt et al. conducted a systematic analysis of LPS-induced gene expression in macrophages.
- The study focused on the dynamics of nascent RNA accumulation and processing.
- Investigated the role of transcription initiation in controlling inflammatory gene expression.
Key Insights:
- Inflammatory responses are primarily governed at the level of transcription initiation.
- Full-length nascent RNAs, including introns, accumulate on chromatin.
- This accumulation facilitates RNA processing before the release of functional messenger RNA (mRNA).
Outlook:
- Further research may elucidate the precise mechanisms of nascent RNA processing on chromatin.
- Understanding this regulatory step could reveal novel therapeutic targets for inflammatory diseases.
- This finding challenges existing models of inflammatory gene expression regulation.
Related Concept Videos
01:10Transcription
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
01:32RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
01:10Transcription
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
01:32RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
01:17Transcription
Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
01:17Transcription
Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...