Isoelectric focusing of cerebrospinal fluid immunoglobulin G: an annotated update

G Keir1, R W Luxton, E J Thompson

  • 1Department of Special Chemical Pathology, National Hospital for Nervous Diseases, Queen Square, London, UK.

A revised agarose isoelectric focusing method for detecting oligoclonal IgG in unconcentrated cerebrospinal fluid is presented. The technique is shown to be robust and reproducible and suitable for the detection of intrathecal IgG synthesis.