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Chitosan/Interfering RNA Nanoparticle Mediated Gene Silencing in Disease Vector Mosquito Larvae
Published on: March 25, 2015
Gene silencing by chemically modified siRNAs
1Goethe-University, Institute of Organic Chemistry and Chemical Biology, Max-von-Laue-Strasse 7, 60438 Frankfurt am Main, Germany. joachim.engels@chemie.uni-frankfurt.de
Abstract:
RNA interference (RNAi) has not only already risen as a gold standard for validating gene function in basic science studies, but also holds great promise as a new therapeutic paradigm. Advantages of RNAi-based therapeutics include relatively fast initial screening and the ability to target proteins not yet addressable by traditional drug design strategies. In this review we describe the development of chemically modified small inhibiting siRNAs and their application as potential therapeutics during the past decade. Focus is on proper siRNA design, choice of chemical modification and how to circumvent immunogenicity as well as off-target effects.
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