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Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis
Published on: August 12, 2019
Denaturation mapping of Saccharomyces cerevisiae
Robert L Welch1, Robert Sladek, Ken Dewar
1Department of Physics, McGill University, 3600 rue University, Montreal, Canada.
Lab on a Chip
|July 24, 2012
Summary
Optical mapping, a technique using DNA denaturation, successfully mapped the yeast genome. This method provides large-scale genomic data for DNA assembly and rearrangement detection.
Area of Science:
- Genomics
- Molecular Biology
- Biophysics
Background:
- Optical mapping of DNA offers large-scale genomic insights for contig assembly and single-cell rearrangement detection.
- Denaturation mapping is an optical mapping technique that utilizes physical principles for genomic structure analysis, avoiding enzymatic methods.
Purpose of the Study:
- To apply denaturation mapping for locating single DNA molecules within the yeast genome.
- To construct an optical map of the yeast genome using denaturation mapping data.
Main Methods:
- Denaturation mapping employed fluorescence microscopy to visualize partial DNA melting patterns.
- DNA molecules were extended within a 120 nm nanofluidic channel.
- Images were compared against a computationally predicted map of the entire yeast genome sequence.
Main Results:
- Successfully located 84 individual DNA molecules on the yeast genome.
- Assembled an optical map of the yeast genome.
- Achieved over 50% coverage of the yeast genome with the optical map.
Conclusions:
- Denaturation mapping is a viable physical method for large-scale genome mapping.
- This technique facilitates the assembly of complex genomes and the identification of genomic rearrangements.
