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Related Experiment Video

Updated: May 20, 2026

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

Published on: February 24, 2015

High-throughput sequencing of the methylome using two-base encoding.

Christina A Bormann Chung¹

¹Life Technologies, Foster City, CA, USA. Christina.Chung@lifetech.com

Methods in Molecular Biology (Clifton, N.J.)

|July 24, 2012

Summary

This summary is machine-generated.

Studying DNA methylation patterns is crucial for understanding cell development and diseases like cancer. This research introduces a new method using bisulfite sequencing and SOLiD technology for precise methylome analysis.

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Targeted DNA Methylation Analysis by Next-generation Sequencing

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Area of Science:

Epigenetics and Molecular Biology
Genomics and Bioinformatics

Background:

Epigenetic modifications, including DNA methylation, are critical for cellular regulation in both health and disease.
Altered methylation patterns are linked to various conditions such as cancer, neurodevelopmental, and endocrine disorders.

Purpose of the Study:

To describe a novel method for analyzing the methylome at single-base resolution.
To enhance the understanding of epigenetic regulation in disease development.

Main Methods:

Utilized bisulfite sequencing to detect methylation.
Combined bisulfite sequencing with high-throughput SOLiD sequencing technology for methylome analysis.

Main Results:

Developed a method enabling single-base resolution of DNA methylation patterns.
Facilitated detailed study of the methylome.

Conclusions:

The described method provides valuable insights into the underlying causes of diseases by analyzing methylation patterns.
High-throughput sequencing technologies are powerful tools for epigenetic research.