Related Experiment Video

Updated: May 20, 2026

Preparation of Pseudo-Typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity
07:15

Preparation of Pseudo-Typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity

Published on: November 22, 2021

H5N1 virus causes significant perturbations in host proteome very early in influenza virus-infected primary human

Chung Yan Cheung1, Eric Y Chan, Alexei Krasnoselsky

  • 1Centre of Influenza Research & School of Public Health, University of Hong Kong.

The Journal of Infectious Diseases
|July 24, 2012
PubMed

Insights

Highly pathogenic H5N1 influenza virus infection significantly alters host cell proteomes early on, distinguishing it from H1N1. H5N1 targets the translational machinery for replication advantage.

Area of Science:

  • Virology
  • Immunology
  • Proteomics

Background:

  • Highly pathogenic H5N1 influenza viruses cause severe human disease.
  • Understanding the host response to influenza infection is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate the temporal host proteome response in human macrophages infected with H5N1 and H1N1 influenza viruses.
  • To identify early host response markers distinguishing H5N1 from H1N1 infection.

Main Methods:

  • Primary human monocyte-derived macrophages were infected with H5N1 and H1N1 influenza viruses.
  • Quantitative proteomic profiling using mass spectrometry was employed to analyze host protein changes.
  • Temporal analysis was performed as early as 1 hour post-infection.

Main Results:

  • Significant perturbation of the host proteome was observed as early as 1 hour after infection.
  • The early host response differed significantly between H5N1 and H1N1 infections, with H5N1 inducing a more pronounced response.
  • The translational machinery was the most affected cellular component, suggesting its exploitation by H5N1.

Conclusions:

  • Early host proteome profiling can distinguish H5N1 from H1N1 influenza virus infections.
  • H5N1 influenza virus may gain a replication advantage by targeting the host's translational machinery early in infection.

More Related Videos

Isolation, Transfection, and Culture of Primary Human Monocytes09:13

Isolation, Transfection, and Culture of Primary Human Monocytes

Published on: December 16, 2019

An In vitro Co-infection Model to Study Plasmodium falciparum-HIV-1 Interactions in Human Primary Monocyte-derived Immune Cells07:39

An In vitro Co-infection Model to Study Plasmodium falciparum-HIV-1 Interactions in Human Primary Monocyte-derived Immune Cells

Published on: August 15, 2012

Related Concept Videos

Influenza01:27

Influenza

Influenza is an acute, highly communicable viral disease that affects the respiratory tract and is responsible for seasonal epidemics worldwide. Influenza A is the most prevalent type associated with widespread outbreaks and is subtyped based on two surface glycoproteins: hemagglutinin (H) and neuraminidase (N), as in H1N1. These glycoproteins are essential for viral infectivity, transmission, and immune recognition. Transmission occurs primarily through respiratory droplets and contaminated...
Viral Mutations00:36

Viral Mutations

A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material for adaptive...
Leaky Scanning02:28

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...
Immune Response Against Viral Pathogens01:29

Immune Response Against Viral Pathogens

The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
Viral Recombination00:57

Viral Recombination

Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
Human Virome01:26

Human Virome

The human body harbors a vast and diverse viral community known as the human virome. The virome includes bacteriophages that infect bacteria, and eukaryotic viruses that infect human cells. Transient dietary and environmental viruses also contribute to this dynamic ecosystem. Estimates suggest the human body may contain on the order of 10¹³ viral particles, though abundance varies widely by body site and detection method.Comprehensive characterization of the virome has become possible only with...