Related Experiment Video
Updated: May 20, 2026

NMR Spectroscopy as a Robust Tool for the Rapid Evaluation of the Lipid Profile of Fish Oil Supplements
Published on: May 1, 2017
Changes in the amino acid composition of Bogue (Boops boops) fish during storage at different temperatures by 1H-NMR
Alessandra Ciampa1, Gianfranco Picone, Luca Laghi
1Department of Food Science, University of Bologna, Cesena 47023 (FC), Italy. alessandra.ciampa2@unibo.it
Abstract:
Nuclear magnetic resonance spectroscopy was employed to obtain information about the changes occurring in Bogue (Boops boops) fish during storage. For this purpose, (1)H-NMR spectra were recorded at 600 MHz on trichloroacetic acid extracts of fish flesh stored over a 15 days period both at 4 °C and on ice. Such spectra allowed the identification and quantification of amino acids, together with the main organic acids and alcohols. The concentration of acidic and basic free amino acids was generally found to increase and decrease during storage, respectively. These concentration changes were slow during the first days, as a consequence of protein autolysis, and at higher rates afterward, resulting from microbial development. Two of the amino acids that showed the greatest concentration change were alanine and glycine, known to have a key role in determining the individual taste of different fish species. The concentration of serine decreased during storage, as highlighted in the literature for frozen fish samples. Differences in the amino acids concentration trends were found to be related to the different storage temperatures from day 4 onwards.
Related Concept Videos
¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR
Atomic Spectroscopy: Effects of Temperature
At thermal equilibrium, the relative populations of excited and ground state atoms can be estimated using the Maxwell–Boltzmann distribution. For example, an increase in temperature from...
¹H NMR of Labile Protons: Temporal Resolution
The –OH proton in alcohols typically appears in the range of δ 2 to 5 ppm but can vary depending on the specific...
NMR Spectroscopy Of Amines
¹H NMR of Conformationally Flexible Molecules: Temporal Resolution
NMR Spectroscopy: Chemical Shift Overview
For instance, the proton...

