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Published on: January 14, 2016
[Callus induction of Liriope muscari and its dynamic accumulation of polysaccharide]
Xue-feng Wan1, Ying-zhen Huang, Zhong-xiong Lai
1Agricultural Bio-resources Institute of Fujian Academy of Agricultural Sciences, Fuzhou 350003, China. wxf125@163.com
Objective:
To study the induction rate of callus tissue in four different explants of stem, root, leaf and fruit of Liriope muscari.
Methods:
The effect of 2,4-D, sugar and illumination on callus succeeding preservation was analyzed. The dynamic accumlation of polysaccharide in callus was described. The polysaccharide content among callus, tissue culture seedings and field seedings was compared.
Results And Conclusion:
The callus induction rate of stem was the highest. The optimal concentration range of 2,4-D was 1.5-2.0 mg/L, then the induction rate was 87.5%. When the 2,4-D conncentration was 0.5-1.0 mg/L, and the sucrose concentration was 20 g/L, the multiplication coefficient was highest. The illumination condition did not influence the effect of callus succeeding preservation. The content of callus polysaccharide continuously increased for 60 d. The growth rate of callus was reached the highest level from 40 d to 60 d. Polysaccharide content in root of tissue culture seeding was higher than that of the field seeding.
