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[Interference of clindamycin in skin infection in rabbits caused by suture threads infected with slime-producing S.
M R Gismondo1, A M Lo Bue, G Chisari
1Istituto di Microbiologia, Università degli Studi di Catania.
Abstract:
The authors have carried out an experimental study to evaluate the interference of sub-inhibitory concentrations of clindamycin on the adherence of slime producing S. epidermidis to surgical nets and its ability to infect rabbits. 80 rabbits divided into 4 groups, (A, B, C, and D), underwent sham operation of the abdominal zone and were sewn up with sterile nets, infected nets and sub MIC of clindamycin equal to 1/8 MIC, respectively. The results obtained stressed a different survival rate 12 days after operation: 95% in group A, 35% in group B; 100% in group C and 95% in group D. This data were confirmed by the results obtained using MES (Cambridge Stereosecan 150 KM2). From our research an excellent clindamycin inhibitory activity on the pathogenicity of slime producing staphylococci emerged.
Insights
Sub-inhibitory clindamycin concentrations significantly reduced Staphylococcus epidermidis slime adherence and infection in rabbits. This demonstrates clindamycin
Area of Science:
- Microbiology
- Infectious Diseases
- Pharmacology
Background:
- Slime-producing Staphylococcus epidermidis is a significant cause of surgical site infections.
- Sub-inhibitory antibiotic concentrations can impact bacterial virulence factors like adherence and biofilm formation.
Purpose of the Study:
- To investigate the effect of sub-inhibitory clindamycin concentrations on the adherence of slime-producing S. epidermidis to surgical nets.
- To evaluate the impact of sub-inhibitory clindamycin on the infectivity of S. epidermidis in a rabbit model.
Main Methods:
- An experimental study involving 80 rabbits divided into four groups.
- Groups were subjected to sham operation, sterile nets, infected nets, or infected nets with sub-minimum inhibitory concentration (MIC) of clindamycin (1/8 MIC).
- Bacterial adherence and rabbit survival rates were assessed 12 days post-operation.
Main Results:
- Survival rates were 95% (sham), 35% (infected nets), 100% (sterile nets), and 95% (clindamycin group).
- Clindamycin treatment significantly improved survival rates compared to untreated infected groups.
- Microscopic examination (MES) confirmed these findings.
Conclusions:
- Sub-inhibitory concentrations of clindamycin effectively inhibit the pathogenicity of slime-producing staphylococci.
- Clindamycin demonstrates significant inhibitory activity against S. epidermidis adherence and infectivity in a surgical context.