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Related Experiment Video

Updated: May 20, 2026

Viability Assays for Cells in Culture

Viability Assays for Cells in Culture

Published on: January 20, 2014

Cell viability assessment: toward content-rich platforms.

Christina Nicole Ramirez¹, Christophe Antczak, Hakim Djaballah

¹HTS Core Facility, Molecular Pharmacology & Chemistry Program, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, NY 10065, USA.

Expert Opinion on Drug Discovery

|July 25, 2012

Summary

Traditional cell viability assays are limited for predicting drug toxicity. High-content, multiplexed readouts offer a more accurate method for assessing cell death and improving drug discovery outcomes.

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Last Updated: May 20, 2026

Viability Assays for Cells in Culture

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Published on: January 20, 2014

Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells

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An Automated Differential Nuclear Staining Assay for Accurate Determination of Mitocan Cytotoxicity

An Automated Differential Nuclear Staining Assay for Accurate Determination of Mitocan Cytotoxicity

Published on: May 12, 2020

Area of Science:

Biomedical research
Drug discovery
Toxicology

Background:

In vitro cell viability monitoring is crucial for predicting in vivo drug toxicology.
High-throughput screening has advanced compound screening but clinical trial failures persist due to toxicity.
Understanding programmed cell death pathways necessitates moving beyond simple endpoint measurements.

Purpose of the Study:

To review traditional cell viability assays and their limitations.
To propose advanced methods for accurate cell death assessment.

Main Methods:

Discussion of commercially available viability assays suitable for high-density formats.
Categorization of assays into dye exclusion, DNA condensation, and metabolic function monitoring.
Emphasis on limitations of current approaches through case studies.

Main Results:

Current single-parameter readouts are prone to errors due to cell heterogeneity and complex cell death mechanisms.
Live, multiplexed readouts are proposed as an alternative for accurate cell death induction recording.

Conclusions:

High-content, continuous, multiplexed readouts are essential for simultaneously monitoring multiple cell death markers.
Accurate prediction of drug candidate toxicology requires content-rich, cell-by-cell analysis platforms.