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Related Concept Videos

Drug Discovery: Overview01:26

Drug Discovery: Overview

Drug discovery is a multifaceted process involving extensive screening, testing, and optimization of lead compounds to identify potential new drugs for therapeutic use. It combines several approaches, including screening large numbers of natural products, chemical modification of known active molecules, identification of new drug targets, and rational design based on biological mechanisms and drug-receptor structure. These approaches are carried out in both academic research laboratories and...
Therapeutic Drug Monitoring: Drug Analysis Methods01:26

Therapeutic Drug Monitoring: Drug Analysis Methods

Therapeutic Drug Monitoring (TDM) is a clinical practice that measures specific drug levels in a patient's blood or body tissues to tailor drug therapy effectively. This monitoring is critical for managing drugs with narrow therapeutic indices like digoxin and phenytoin, ensuring they are both safe and effective. For instance, monitoring theophylline levels in asthma patients involves precision and sensitivity to adjust doses according to individual responses to therapy, ensuring efficacy and...
Pharmacogenetics of Drug Metabolism: Overview01:27

Pharmacogenetics of Drug Metabolism: Overview

Genetic polymorphism in drug metabolism is crucial to the inter-individual variability observed in drug responses. Drug metabolism primarily involves the chemical modification of drugs and other xenobiotics to enhance their elimination by increasing their polarity. Two main classes of enzymes mediate this biotransformation process: Phase I enzymes, primarily cytochrome P450s, catalyze oxidation and reduction reactions, while other enzymes, such as esterases, mediate hydrolysis, and Phase II...
Pharmacogenomics: Identification of New Drug Targets01:29

Pharmacogenomics: Identification of New Drug Targets

Advances in genomics have profoundly influenced drug discovery by increasing both the speed and accuracy of pharmaceutical development. Pharmacogenomics, which examines how genetic variation influences drug response, facilitates the identification of novel therapeutic targets and enables patient stratification for personalized treatment. These strategies contribute to improved drug efficacy, minimized adverse effects, and more efficient clinical trial design.Mapping genetic differences...
Measurement of Bioavailability: Pharmacodynamic Methods01:20

Measurement of Bioavailability: Pharmacodynamic Methods

Pharmacodynamic methods provide insights into a drug's effects on physiological processes over time and play a crucial role in understanding bioavailability and therapeutic efficacy. These methods can be broadly classified into acute pharmacological and therapeutic response approaches, each with distinct mechanisms and applications.The acute pharmacological response method directly correlates a drug's physiological effects, such as ECG or pupil diameter changes, to its time course in the body.

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Updated: May 20, 2026

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

Practical metabolomics in drug discovery.

Keith M Wilcoxen1, Taisuke Uehara, Khin Than Myint

  • 1Eisai, Inc., Biomarkers and Personalized Medicine, 4 Corporate Drive, Andover, MA 01810, USA +1 978 837 4614 ; +1 978 837 4863 ; Keith_Wilcoxen@eisai.com.

Expert Opinion on Drug Discovery
|July 25, 2012
PubMed
Summary

Metabolomics enables comprehensive profiling of molecules for drug development. This review covers practical experimental design, sample preparation, and analysis methods for mammalian biological systems.

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Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics

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Area of Science:

  • Biochemistry
  • Pharmacology
  • Analytical Chemistry

Background:

  • Metabolomics is crucial in pharmaceutical research for discovering and developing therapeutics.
  • It offers simultaneous profiling of numerous molecules (amino acids, sugars, lipids) in biological samples.
  • This 'omics' field bridges internal biology and external influences on disease.

Purpose of the Study:

  • To provide an overview of metabolomics in drug development.
  • To discuss practical experimental design considerations.
  • To detail sample preparation and analysis for various metabolites.

Main Methods:

  • Focuses on experimental design for metabolomics studies in mammalian systems.
  • Covers sample preparation and analysis techniques for both polar and non-polar metabolites.
  • Highlights recent advancements in metabolomic methodologies.

Main Results:

  • The review offers insights into metabolomics applications in drug development.
  • It details practical aspects of experimental design, sample handling, and data analysis.
  • Recent advances in analytical techniques are presented.

Conclusions:

  • Metabolomics is a rapidly growing field essential for drug development.
  • Diverse metabolites necessitate varied analytical approaches.
  • The field will increasingly impact disease characterization and biomarker development for drug efficacy and safety.