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Updated: May 20, 2026
10:24
Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
Published on: August 29, 2014
|SE|S|AM|E| Barcode: NGS-oriented software for amplicon characterization--application to species and environmental
1INRA (UMR CBGP Centre de Biologie Pour la Gestion des Populations), Campus international de Baillarguet, Montferrier-sur-Lez Cedex, France.
Molecular Ecology Resources
|July 25, 2012
Summary
SESAME Barcode offers a unified platform for analyzing next-generation sequencing (NGS) data, simplifying biodiversity characterization for both specimen identification and environmental barcoding. This tool enhances data analysis for biologists using NGS technologies.
Area of Science:
- Genomics
- Bioinformatics
- Ecology
Background:
- Next-generation sequencing (NGS) technologies have significantly advanced biodiversity assessment.
- Current challenges include a lack of user-friendly tools for analyzing the increasing volume of NGS data.
- Biodiversity characterization relies on accurate specimen identification and environmental barcoding.
Purpose of the Study:
- To introduce SESAME Barcode, a unified platform designed to facilitate NGS data analysis.
- To provide a user-friendly solution for processing and analyzing biodiversity data from NGS.
- To support both individual specimen identification and environmental barcoding applications.
Main Methods:
- Development of a bioinformatics pipeline for processing NGS amplicon runs.
- Implementation of steps for filtering markers and samples.
- Creation of reference libraries for sequence identification (barcoding).
Main Results:
- Validation using simulated data for specimen identification.
- Validation using a published dataset for environmental barcoding.
- Obtained results align with expected in silico and previously published characterizations.
Conclusions:
- SESAME Barcode provides a robust and unified platform for NGS data analysis in biodiversity studies.
- The tool effectively supports specimen identification and environmental barcoding.
- SESAME Barcode is freely available, promoting wider accessibility and application in research.
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