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Related Experiment Video

Updated: May 20, 2026

Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection
07:51

Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection

Published on: February 1, 2022

Graphene-based aptamer logic gates and their application to multiplex detection.

Li Wang1, Jinbo Zhu, Lei Han

  • 1State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.

ACS Nano
|July 25, 2012
PubMed
Summary
This summary is machine-generated.

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A novel graphene oxide/aptamer system enables multiplex logic operations for sensing multiple targets. This system regulates fluorescence, paving the way for advanced DNA circuits and biosensors.

Area of Science:

  • Nanomaterials Science
  • Biotechnology
  • Molecular Engineering

Background:

  • Graphene oxide (GO) is a versatile nanomaterial with unique optical and electronic properties.
  • Aptamers are short DNA or RNA sequences that can bind to specific targets with high affinity and specificity.
  • Developing efficient multiplex sensing platforms is crucial for detecting multiple analytes in complex biological samples.

Purpose of the Study:

  • To construct a graphene oxide/aptamer (GO/aptamer) system for multiplex logic operations.
  • To enable the sensing of multiple target molecules simultaneously using programmable logic gates.
  • To demonstrate the potential of this system in developing advanced DNA circuits and biosensors.

Main Methods:

  • Adsorption of 6-Carboxyfluorescein (FAM)-labeled adenosine triphosphate binding aptamer (ABA) and FAM-labeled thrombin binding aptamer (TBA) onto graphene oxide (GO).

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Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
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Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Related Experiment Videos

Last Updated: May 20, 2026

Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection
07:51

Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection

Published on: February 1, 2022

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

  • Utilizing the fluorescence quenching of FAM by GO to create a responsive sensing platform.
  • Implementing OR and INHIBIT logic gates to control FAM fluorescence based on target presence and input combinations.

    • Main Results:

    • A GO/aptamer complex was successfully constructed, leading to fluorescence quenching of FAM.
    • The system demonstrated programmable regulation of FAM fluorescence via OR and INHIBIT logic gates.
    • High-throughput fluorescence imaging was achieved, enabling simultaneous processing of multiple samples using combinatorial logic gates.

    Conclusions:

    • The developed GO/aptamer system effectively integrates OR and INHIBIT logic gates for multiplex logic sensing.
    • This approach offers a promising strategy for identifying multiple targets in complex chemical environments.
    • The logic gates developed may find applications in the advancement of DNA circuits and sophisticated biosensor design.