Related Experiment Video
Updated: May 20, 2026
Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis
Published on: February 6, 2021
Clinical course, immune response, and histopathology of a simplified model of experimental lens induced
T Takagi1, A Nakajima, K Yamaki
1Department of Ophthalmology, Akita University School of Medicine, 1-1-1, Hondo, Akita, 010, Japan.
Abstract:
YAG laser capsulotomy 14 days after one-shot immunization of bovine lens soluble protein with Freund's complete adjuvant and simultaneous intravenous injection of Bordettella pertussis caused lens induced endophthalmitis (LIE) in Lewis rats. Exudate first appeared in the anterior chamber eight hours after capsulotomy. In the cases of low dose (20μg/rat) immunization, exudative change was localized around the ruptured lens surface. On the other hand, the anterior chamber was filled with thick exudate in high dose (200μg/rat) immunized rats. By ELISA and lymphocyte proliferation assay, serum and lymphocyte from LIE rats reacted with bovine uvea as well as bovine lens, but showed no cross-reactivity with bovine retina. Histopathological finding in the low-dose immunized rats was granulomatous inflammation localized to the anterior eye segment, but high dose-immunized rats developed severe panophthalmitis and showed epithelioid granulomas in disorganized retina. The authors think this low dose model can contribute to therapeutic or suppressive studies.
