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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications
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Advances in flow cytometry for drug screening.

Jean Peluso1, Christian D Muller

  • 1Laboratoire d'Innovation Thérapeutique, Faculté de Pharmacie, Université de Strasbourg, UMR CNRS 7200, 74, Route du Rhin, B.P. 24, 67401 Illkirch, Cedex, France +33 3 68 85 42 71 ; +33 3 68 85 43 10 ; cdmuller@unistra.fr.

Expert Opinion on Drug Discovery
|July 25, 2012
PubMed
Summary

Flow cytometry is a mature technology now increasingly used for screening. New analysis software is crucial for advancing its role in drug discovery by enabling multi-parameter, multi-cell type analysis.

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Area of Science:

  • Biotechnology
  • Drug Discovery
  • Cell Biology

Background:

  • Flow cytometry is a well-established technology with recent advancements making it suitable for screening applications.
  • Despite its potential, flow cytometry is underutilized for drug screening, with limited published studies beyond multiplex bead assays.

Purpose of the Study:

  • To explore the potential of flow cytometry as a primary tool for drug discovery screening.
  • To identify emerging trends and challenges in utilizing flow cytometry for high-throughput screening.

Main Methods:

  • Literature review focusing on technological advancements in flow cytometry for screening.
  • Market survey of new instruments, kits, and methodologies in flow cytometry.
  • Analysis of the role of cytomics in drug discovery.

Main Results:

  • The increasing ease of use of flow cytometry machines necessitates the development of user-friendly, plug-and-analyze software.
  • New analytical tools are essential for simultaneous multi-parameter analysis across multiple cell types and multi-well formats.
  • Cytomics, a multiparametric and dynamic approach to cell research, offers practical applications in drug discovery.

Conclusions:

  • Advanced software with multi-well comparison and real-time cytogram interaction can overcome limitations of fluorescent channels in smaller flow cytometry instruments.
  • Flow cytometry, supported by enhanced analytical software, holds significant promise for future drug discovery.