Related Experiment Video
Updated: May 20, 2026
Fluorescent Orthotopic Mouse Model of Pancreatic Cancer
Published on: September 20, 2016
Orthotopic mouse models expressing fluorescent proteins for cancer drug discovery
1AntiCancer, Inc., 7917 Ostrow Street, San Diego, CA 92111, USA +1 858 654 2555 ; +1 858 268 4175 ; all@anticancer.com.
Importance Of The Field:
Currently used rodent tumor models, including transgenic tumor models, or subcutaneously growing human tumors in immunodeficient mice, do not sufficiently represent clinical cancer, especially with regard to metastasis and drug sensitivity.
Areas Covered In This Review:
To obtain clinically accurate models, we have developed the technique of surgical orthotopic implantation (SOI) to transplant histologically intact fragments of human cancer, including tumors taken directly from the patient, to the corresponding organ of immunodeficient rodents. SOI allows the growth and metastatic potential of the transplanted tumors to be expressed and reflects clinical cancer of all types. Effective drugs can be discovered and evaluated in the SOI models utilizing human tumor cell lines and patient tumors. Visualization of many aspects of cancer initiation and progression in vivo has been achieved with fluorescent proteins. Tumors and metastases in the SOI models that express fluorescent proteins can be visualized noninvasively in intact animals, greatly facilitating drug discovery.
What The Reader Will Gain:
This review will provide information on the imageable mouse models of cancer that are clinically relevant, especially regarding metastasis and their use for drug discovery and evaluation.
Take Home Message:
SOI mouse models of cancer reproduce the features of clinical cancer.
Insights
Surgical orthotopic implantation (SOI) mouse models accurately mimic human clinical cancer, including metastasis and drug response. These imageable models are crucial for effective cancer drug discovery and evaluation.
Area of Science:
- Oncology
- Translational Research
- Preclinical Cancer Models
Background:
- Current rodent models inadequately represent human cancer, particularly metastasis and drug sensitivity.
- Transgenic and subcutaneous xenograft models show limitations in clinical accuracy.
- There is a critical need for more relevant preclinical cancer models.
Purpose of the Study:
- To introduce and review the surgical orthotopic implantation (SOI) technique for creating clinically relevant cancer models.
- To highlight the utility of SOI models for drug discovery and evaluation.
- To discuss the application of imageable models for in vivo cancer research.
Main Methods:
- Surgical orthotopic implantation (SOI) of histologically intact human tumor fragments into corresponding organs of immunodeficient rodents.
- Utilization of human tumor cell lines and patient-derived tumors within SOI models.
- Incorporation of fluorescent proteins for non-invasive in vivo visualization of tumor growth and metastasis.
Main Results:
- SOI models enable the expression of tumor growth and metastatic potential, mirroring clinical cancer.
- These models facilitate the discovery and evaluation of effective anti-cancer drugs.
- Imageable SOI models allow for non-invasive monitoring of cancer initiation, progression, and response to therapy.
Conclusions:
- Surgical orthotopic implantation (SOI) mouse models effectively reproduce key features of clinical human cancer.
- SOI models offer a powerful platform for advancing cancer research and therapeutic development.
- These models are essential for improving the accuracy of preclinical drug testing.
More Related Videos
12:24Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
Published on: July 17, 2012
09:52An Orthotopic Glioblastoma Mouse Model Maintaining Brain Parenchymal Physical Constraints and Suitable for Intravital Two-photon Microscopy
Published on: April 21, 2014
Related Concept Videos
02:43Mouse Models of Cancer Study
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
02:43Mouse Models of Cancer Study
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...