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Updated: May 20, 2026

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Multiplexed protein detection using an affinity aptamer amplification assay.

Kris P F Janssen¹, Karel Knez, Dragana Spasic

¹BIOSYST-MeBioS, KU Leuven, Leuven, Belgium.

Analytical and Bioanalytical Chemistry

|July 25, 2012

Summary

This summary is machine-generated.

Aptamer-enhanced affinity probe capillary electrophoresis (APCE) enables ultrasensitive, multiplexed protein biomarker detection. This advanced technique significantly improves detection limits for protein diagnostics, offering a high-throughput proteomics platform.

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Area of Science:

Biotechnology
Analytical Chemistry
Molecular Diagnostics

Background:

Affinity probe capillary electrophoresis (APCE) is a versatile protein diagnostics technology balancing robustness, speed, and sensitivity.
Traditional APCE faces limitations in ultrasensitive and multiplexed detection of protein biomarkers, especially with similar electrophoretic mobilities.

Purpose of the Study:

To enhance the analytical capabilities of APCE for ultrasensitive and multiplexed detection of protein biomarkers.
To demonstrate the feasibility of using aptamers with nucleic acid amplification to improve APCE performance.

Main Methods:

Integration of aptamers with capillary electrophoresis (CE) and nucleic acid amplification (PCR).
Modification of aptamers for successful PCR amplification.
Optimization of experimental parameters for simultaneous detection of multiple proteins.

Main Results:

Achieved simultaneous detection of picomolar levels of three target proteins.
Demonstrated limits of detection orders of magnitude lower than traditional CE-based immunosensing.
Showcased successful multiplexed detection despite varying aptamer selection conditions and limited mobility differences.

Conclusions:

Aptamer-enhanced APCE offers a significant advancement in protein diagnostics, providing ultrasensitive and multiplexed detection capabilities.
This technology has the potential to become a high-throughput proteomics platform, comparable to genetic screening.
Recent developments in aptamer selection further solidify its role as an accessible, high-affinity recognition tool.