Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

IR Spectrometers

IR Spectrometers

There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...

IR Frequency Region: Fingerprint Region

IR Frequency Region: Fingerprint Region

IR spectra are divided into two main regions: the diagnostic region and the fingerprint region. The diagnostic region of the spectrum lies above 1500 cm−1. The absorptions resulting from single-bond vibrations of the N–H, C–H, and O–H stretch at higher wavenumbers and appear on the left side of the spectrum. The stretching absorptions of the C≡C and C≡N occur between 2100–2300 cm−1. In contrast, those arising from stretching absorptions of the C=O, C=N, and C=C occur between 1600–1850 cm−1.
The...

Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

Infrared (IR) Spectroscopy: Overview

Infrared (IR) Spectroscopy: Overview

When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
Different compounds display unique properties due to their...

IR Spectroscopy: Molecular Vibration Overview

IR Spectroscopy: Molecular Vibration Overview

When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...

Applications of IR Spectroscopy: Overview

Applications of IR Spectroscopy: Overview

The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Production of gamma-polyglutamic acid with tunable molecular weight via electrofermentation using soybean protein concentrate as a feedstock.

Bioresource technology·2026

Same author

Progranulin enhances complement component 5a-primed neutrophil activation in antineutrophil cytoplasmic antibody-associated vasculitis.

Immunology letters·2026

Same author

High-Capacityand Reversible Hydrogen Storage in an Intrinsic Li<sub>3</sub>B<sub>2</sub>N<sub>2</sub> Monolayer.

Nanomaterials (Basel, Switzerland)·2026

Same author

Mechanical Programming of Carrier Flow by Band-Alignment Inversion in Two-Dimensional Heterostructures.

Nano letters·2026

Same author

MscaVPR: Multi-Scale Coordinate Attention Network for Robust Visual Place Recognition.

Sensors (Basel, Switzerland)·2026

Same author

Effect of transcutaneous electrical acupoint stimulation on major complications after cerebral endovascular procedures (ASSET): study protocol for a randomized controlled trial.

International journal of surgery protocols·2026

Search research articles

Related Experiment Video

Updated: May 20, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

[Phase correction technology research and improvement based on single-sided interferograms in FTIR].

Min-juan Zhang¹, Ji-long Zhang, Zhi-bin Wang

¹Key Laboratory of Instrumentation Science & Dynamic Measurement (North University of China), Ministry of Education, Taiyuan 030051, China. zmj7745@163.com

Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu

|July 26, 2012

Summary

This study improves phase correction in Fourier transform infrared spectroscopy by combining Mertz and Forman methods. The new approach enhances spectral resolution and accuracy while reducing computational load.

More Related Videos

High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

Published on: January 21, 2015

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

Published on: June 27, 2014

Related Experiment Videos

Last Updated: May 20, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

Published on: January 21, 2015

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

Published on: June 27, 2014

Area of Science:

Spectroscopy
Optical Engineering
Signal Processing

Context:

Fourier transform infrared (FTIR) spectrometers often use single-sided interferograms, leading to asymmetry.
Existing phase correction methods like Mertz can reduce spectral resolution due to asymmetric apodization.

Purpose:

To develop an improved phase correction technique for FTIR spectroscopy.
To address limitations of existing methods concerning spectral resolution and computational efficiency.

Summary:

A novel method constructs a double-sided interferogram from a single-sided one, enabling symmetric apodization and accurate phase correction.
This technique combines aspects of the Mertz and Forman methods, utilizing mirrored processing and symmetrization for high-resolution phase spectra.
The improved method achieves a spectral resolution of 2 cm⁻¹ with reduced error and computation compared to traditional approaches.

Impact:

Enhances the accuracy and resolution of spectral data obtained from FTIR spectrometers.
Offers a more efficient and precise alternative for phase correction in spectroscopic analysis.
Facilitates higher quality spectral measurements in various scientific and industrial applications.