Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Oogenesis02:07

Oogenesis

In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...
Oogenesis01:22

Oogenesis

Oogenesis,  the process of developing egg cells (female gametes), occurs within the ovaries and is fundamental to female fertility. This sequence begins during fetal development when diploid oogonia in the developing ovaries undergo mitotic divisions to produce primary oocytes. By birth, these primary oocytes enter prophase I of meiosis but become arrested in this stage, remaining suspended until puberty.
Each primary oocyte is surrounded by a layer of pre-granulosa cells, forming what is known...
General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Epigenetic signature of prenatal heat stress: DNA methylation changes in whole blood of dairy calves from birth to weaning.

Journal of dairy science·2025
Same author

Transcriptome and epigenome analysis of porcine embryos from non-esterified fatty acid-exposed oocytes.

Domestic animal endocrinology·2021
Same author

The influence of in vitro fertilization and embryo culture on the embryo epigenetic constituents and the possible consequences in the bovine model.

Journal of developmental origins of health and disease·2017
Same author

Effects of intramuscular administration of folic acid and vitamin B12 on granulosa cells gene expression in postpartum dairy cows.

Journal of dairy science·2015
Same author

Analysis of microRNAs and their precursors in bovine early embryonic development.

Molecular human reproduction·2012
Same author

Effect of ovarian stimulation on oocyte gene expression in cattle.

Theriogenology·2012
Same journal

Immuno-Targeting of CLRN3 and SCAMP1 as a Potential Sex Specific Marker in Bovine Spermatozoa.

Reproduction in domestic animals = Zuchthygiene·2026
Same journal

Effect of BRD0539 on Gene Editing and Mosaicism Rate in Porcine Gene Editing Embryos by CRISPR/Cas9.

Reproduction in domestic animals = Zuchthygiene·2026
Same journal

Enhancement of Ram Sperm Quality During Chilled Storage by Supplementation With Spirulina platensis Extract.

Reproduction in domestic animals = Zuchthygiene·2026
Same journal

Associations of Management Factors and Environmental Conditions With the Number of Liveborn Piglets in a Commercial Pig Farm: A Retrospective Field Study.

Reproduction in domestic animals = Zuchthygiene·2026
Same journal

Y-Sperm Enrichment Through TLR 7/8 Validated Through Molecular and Biochemical Approaches in Sahiwal Bull.

Reproduction in domestic animals = Zuchthygiene·2026
Same journal

Evaluation of Proliferative Potential and Collagen Deposition in Vitrified Canine Testicular Fragments Subjected to Different Cryoprotectant Combinations and Warming Temperatures.

Reproduction in domestic animals = Zuchthygiene·2026
See all related articles

Related Experiment Video

Updated: May 20, 2026

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay
08:00

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Published on: June 16, 2021

Factors affecting oocyte and embryo transcriptomes.

M-A Sirard1

  • 1Centre de Recherche en Biologie de la Reproduction, Faculté des Sciences de l'Agriculture et de l'Alimentation, Pavillon des Services, Université Laval, Québec City, QC, Canada. sirard@fsaa.ulaval.ca

Reproduction in Domestic Animals = Zuchthygiene
|July 26, 2012
PubMed
Summary
This summary is machine-generated.

Oocyte preparation by the follicle is crucial for early embryo development, influencing the maternal transcriptome. Understanding maternal mRNA storage, degradation, and embryonic genome activation is key to optimizing oocyte quality and in vitro fertilization success.

More Related Videos

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes
12:11

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Published on: May 11, 2017

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing
09:16

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

Published on: October 11, 2015

Related Experiment Videos

Last Updated: May 20, 2026

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay
08:00

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Published on: June 16, 2021

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes
12:11

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Published on: May 11, 2017

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing
09:16

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

Published on: October 11, 2015

Area of Science:

  • Reproductive Biology
  • Developmental Biology
  • Genetics

Background:

  • The oocyte's transcriptome is significantly influenced by the preceding follicular environment before meiotic resumption.
  • Maternal factors stored within the oocyte dictate development up to the 8-cell stage.
  • Properly prepared oocytes can lead to high blastocyst rates in vitro, highlighting the importance of intra-oocyte mechanisms.

Purpose of the Study:

  • To review the critical period from full-size oocytes to the 8-cell stage.
  • To summarize the impact of follicular environment, maternal RNA storage, and embryonic genome activation on the oocyte/early embryo transcriptome.
  • To explore the mechanisms underlying oocyte preparation and maternal mRNA regulation.

Main Methods:

  • Literature review focusing on oocyte maturation and early embryonic development.
  • Analysis of factors influencing the maternal transcriptome, including mRNA storage, degradation, and poly(A) tail length.
  • Discussion of the potential role of microRNAs (miRNAs) in maternal mRNA depletion.

Main Results:

  • The follicular environment's legacy is the primary determinant of the oocyte and early embryo transcriptome.
  • Maternal mRNA storage and degradation, influenced by factors like poly(A) tail length and potentially miRNAs, are critical.
  • Embryonic genome activation is linked to maternal mRNA degradation, alongside other developmental processes.

Conclusions:

  • Optimal oocyte quality depends on precise follicular timing and differentiation, though intra-oocyte mechanisms require further elucidation.
  • Understanding maternal RNA dynamics and embryonic genome activation is essential for improving in vitro fertilization outcomes.
  • Further research into the molecular mechanisms governing oocyte preparation and maternal factor regulation is warranted.