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Analysis of herpes virus group (DNA) from cerebrospinal fluid in vogt-koyanagi-harada disease

Y Hotta1, M Hayakawa, H Kawano

  • 1Department of Ophthalmology, Juntendo University School of Medicine, Hongo, Bunkyo-ku, Tokyo, 113.

Ocular Immunology and Inflammation
|July 26, 2012
PubMed

Insights

This study investigated herpes virus DNA in Vogt-Koyanagi-Harada (VKH) disease patients using PCR on cerebrospinal fluid. No herpes virus DNA was detected, suggesting no close association between this virus group and VKH disease.

Area of Science:

  • Ophthalmology
  • Neurology
  • Virology

Background:

  • Vogt-Koyanagi-Harada (VKH) disease is a rare multisystem inflammatory disorder.
  • The etiology of VKH disease remains unclear, with viral infections being a suspected cause.

Purpose of the Study:

  • To detect herpes virus group DNA, including Epstein-Barr virus (EBV), in patients diagnosed with Vogt-Koyanagi-Harada (VKH) disease.
  • To investigate the potential association between herpes virus infections and the pathogenesis of VKH disease.

Main Methods:

  • Polymerase chain reaction (PCR) was utilized to amplify DNA fragments.
  • Cerebrospinal fluid (CSF) samples from six patients with active VKH disease were analyzed.
  • PCR targeted sequences of herpes simplex virus (HSV), herpes zoster virus (VZV), cytomegalovirus (CMV), EBV, and human herpes virus type 6 (HHV-6).

Main Results:

  • No DNA fragments specific to the herpes virus group were identified in the analyzed CSF samples.
  • The study found no evidence of herpes simplex virus, herpes zoster virus, cytomegalovirus, Epstein-Barr virus, or human herpes virus type 6 DNA.

Conclusions:

  • The findings do not support a close association between the herpes virus group and the development of Vogt-Koyanagi-Harada disease.
  • Further research may be needed to explore other potential etiologies for VKH disease.

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