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Related Experiment Videos

Calibration and control in haemoglobinometry.

O W van Assendelft1, B R Horton, R M Parvin

  • 1Clinical Medicine Branch, Centers for Disease Control, Atlanta, Georgia.

Clinical and Laboratory Haematology
|January 1, 1990
PubMed
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Accurate hemoglobin concentration measurement is crucial for calibrating blood cell analyzers. The International Council for Standardization in Hematology (ICSH) hemiglobincyanide (HiCN) method is recommended, but errors can occur during collection, dilution, or measurement.

Area of Science:

  • Hematology
  • Clinical Chemistry

Background:

  • Automated blood cell analyzers require accurate calibration using fresh blood specimens.
  • Hemoglobin concentration is a critical parameter for blood analysis.

Purpose of the Study:

  • To highlight potential errors in the ICSH recommended hemiglobincyanide (HiCN) method for hemoglobin determination.
  • To compare commercial reagent systems and secondary standards with ICSH standards.

Main Methods:

  • Review of potential error sources in the hemiglobincyanide (HiCN) method.
  • Comparison of commercial reagent systems and secondary standards against ICSH standards.

Main Results:

  • Identified potential errors in blood collection, dilution, reaction completion, and measurement.

Related Experiment Videos

  • Evaluated compliance of commercial systems with ICSH standards.

    • Conclusions:

    • Adherence to the ICSH hemiglobincyanide (HiCN) method is vital for accurate hemoglobin calibration.
    • Awareness of potential errors is necessary for reliable blood analyzer calibration.