Related Experiment Video
Updated: May 20, 2026
Preparation of Functional Silica Using a Bioinspired Method
Published on: August 1, 2018
Effects of silica nanoparticle supported ionic liquid as additive on thermal reversibility of human carbonic
Azadeh Fallah-Bagheri1, Ali Akbar Saboury, Leila Ma'mani
1Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
Abstract:
Silica nanoparticle supported imidazolium ionic liquid [SNImIL] was synthesized and utilized as a biocompatible additive for studying the thermal reversibility of human carbonic anhydrase II (HCA II). For this purpose, we prepared additive by modification of nanoparticles through the grafting of ionic liquids on the surface of nanoparticles (SNImIL). The SNImIL were fully characterized by Fourier transform infrared spectroscopy, scanning electron microscopy and thermo gravimetric analysis. The characterization of HCA II was investigated by various techniques including UV-vis and ANS fluorescence spectrophotometry, differential scanning calorimetry, and docking study. SNImIL induced disaggregation, enhanced protein stability and increased thermal reversibility of HCA II by up to 42% at pH 7.75.
